Synchronous Sterilization and Immunoreaction Termination for Corneal Transparency Protection in Treating Pseudomonas aeruginosa Induced Bacterial Keratitis

Détails bibliographiques
Publié dans:	Advanced materials (Deerfield Beach, Fla.). - 1998. - 37(2025), 20 vom: 01. Mai, Seite e2419209
Auteur principal:	Qian, Siyuan (Auteur)
Autres auteurs:	Wang, Xuan, Guo, Yishun, He, Wenfang, Yang, Jianhua, Chen, Hao, Li, Renlong, Su, Lili, Wang, Xinyi, Shao, Yi, Wang, Bailiang
Format:	Article en ligne
Langue:	English
Publié:	2025
Accès à la collection:	Advanced materials (Deerfield Beach, Fla.)
Sujets:	Journal Article endotoxin neutralization infectious keratitis lipopolysaccharide proinflammatory cytokines sterilization Polymyxin B J2VZ07J96K Anti-Bacterial Agents Silicon Dioxide plus... 7631-86-9 Lipopolysaccharides Carbon 7440-44-0 Cytokines


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245	1	0	\|a Synchronous Sterilization and Immunoreaction Termination for Corneal Transparency Protection in Treating Pseudomonas aeruginosa Induced Bacterial Keratitis
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520			\|a In the treatment of infectious keratitis, therapeutic strategies often prioritize enhancing bactericidal efficacy. However, endotoxins released from Gram-negative bacteria cause inflammatory reaction, leading to corneal structural damage and scar formation. Given that polymyxin B (PMB) can bind and neutralize lipopolysaccharide (LPS), this study employs large-pore mesoporous silica nanoparticles (lMSNs) grafted with PMB as carriers for cationic antibacterial carbon quantum dots (CQDs) to prepare CQDlMSN-PMB, which enables synchronous sterilization and endotoxin neutralization. In the acidic infectious microenvironment, the accelerated release of CQDs eliminates 99.88% bacteria within 2 h, effectively substituting immune mediated sterilization. Notably, CQD@lMSN-PMB exhibits exceptional LPS neutralization performance (2.22 µg LPS/mg CQD@lMSN-PMB) due to its high specific surface area. In an infectious keratitis model, inflammation subsides significantly within the first day of CQD@lMSN-PMB intervention and is completely resolved by day 3. By day 2, interleukin-1β, interleukin-6 and tumor necrosis factor-α in CQD@lMSN-PMB group decrease by 86.99%, 91.15%, and 77.56%, respectively, compared to the CQDs-only sterilization group. Ultimately, corneal integrity and transparency are preserved, with suppressed expressions of fibrosis-related factors including matrix metalloproteinase 9, transforming growth factor-β and α-smooth muscle actin. Therefore, this synchronous sterilization and endotoxin neutralization strategy outperforms monotherapy strategies focused solely on sterilization or endotoxin neutralization
650		4	\|a Journal Article
650		4	\|a endotoxin neutralization
650		4	\|a infectious keratitis
650		4	\|a lipopolysaccharide
650		4	\|a proinflammatory cytokines
650		4	\|a sterilization
650		7	\|a Polymyxin B \|2 NLM
650		7	\|a J2VZ07J96K \|2 NLM
650		7	\|a Anti-Bacterial Agents \|2 NLM
650		7	\|a Silicon Dioxide \|2 NLM
650		7	\|a 7631-86-9 \|2 NLM
650		7	\|a Lipopolysaccharides \|2 NLM
650		7	\|a Carbon \|2 NLM
650		7	\|a 7440-44-0 \|2 NLM
650		7	\|a Cytokines \|2 NLM
700	1		\|a Wang, Xuan \|e verfasserin \|4 aut
700	1		\|a Guo, Yishun \|e verfasserin \|4 aut
700	1		\|a He, Wenfang \|e verfasserin \|4 aut
700	1		\|a Yang, Jianhua \|e verfasserin \|4 aut
700	1		\|a Chen, Hao \|e verfasserin \|4 aut
700	1		\|a Li, Renlong \|e verfasserin \|4 aut
700	1		\|a Su, Lili \|e verfasserin \|4 aut
700	1		\|a Wang, Xinyi \|e verfasserin \|4 aut
700	1		\|a Shao, Yi \|e verfasserin \|4 aut
700	1		\|a Wang, Bailiang \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Advanced materials (Deerfield Beach, Fla.) \|d 1998 \|g 37(2025), 20 vom: 01. Mai, Seite e2419209 \|w (DE-627)NLM098206397 \|x 1521-4095 \|7 nnas
773	1	8	\|g volume:37 \|g year:2025 \|g number:20 \|g day:01 \|g month:05 \|g pages:e2419209
856	4	0	\|u http://dx.doi.org/10.1002/adma.202419209 \|3 Volltext
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