The Mechanism of Nitrite Reductase
© 2025 The Author(s). Journal of Computational Chemistry published by Wiley Periodicals LLC.
| Publié dans: | Journal of computational chemistry. - 1984. - 46(2025), 8 vom: 30. März, Seite e70088 |
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| Auteur principal: | |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Journal of computational chemistry |
| Sujets: | Journal Article DFT cytochrome c nitrite reductase mechanisms quantum chemistry redox enzymes nitrate reductase (cytochrome) EC 1.9.6.1 Nitrite Reductases EC 1.7.- plus... |
| Résumé: | © 2025 The Author(s). Journal of Computational Chemistry published by Wiley Periodicals LLC. Cytochrome c nitrite reductase (CcNiR) activates nitrite and produces ammonia. It is one of several enzymes that use a redox-active cofactor to perform its reaction. In this case, the cofactor has a heme with a lysine as the proximal ligand and a charged nearby arginine. The role of a tyrosine, which is also close, has been less clear. There are also four bis-histidine-ligated hemes involved in the electron transfers. CcNiR has been studied before, using essentially the same methods as here. However, the mechanism is very complicated, involving six reductions, and quite different results for the mechanism have been obtained here. For example, the tyrosine has here been found to be redox active in the final step when ammonia is produced. Also, the arginine has here been found to stay protonated throughout the mechanism, which is different from what was found in the previous study. The present results are in very good agreement with experimental findings and are, therefore, another case where the methodology has been shown to work very well. Previous examples include Photosystem II and Nitrogenase, normally considered to be the most important enzymes in nature for the development of life |
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| Description: | Date Completed 24.03.2025 Date Revised 27.03.2025 published: Print Citation Status MEDLINE |
| ISSN: | 1096-987X |
| DOI: | 10.1002/jcc.70088 |