Non-CG DNA methylation represses SDC expression to modulate hypocotyl elongation during thermormorphogenesis
© The Author(s) 2025. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink serv...
| Publié dans: | Journal of experimental botany. - 1985. - (2025) vom: 10. März |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Journal of experimental botany |
| Sujets: | Journal Article Arabidopsis thaliana DNA methylation PIF4 SDC auxin metabolism epigenetics hypocotyl elongation thermomorphogenesis |
| Résumé: | © The Author(s) 2025. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. Plants adapt to warm environments through physiological and morphological changes termed thermomorphogenesis, which involve transcriptional reprogramming exerted mainly by PHYTOCHROME INTERACTING FACTOR 4 (PIF4). Fluctuating temperatures can also influence the patterns of cytosine DNA methylation, thereby influencing gene expression. However, whether these epigenetic changes provide an adaptative advantage remains unclear. Here, we provide evidence that DNA methylation is required to regulate thermomorphogenesis. Hypomethylated drm1 drm2 cmt3 mutants or seedlings treated with 5-azacytidine to block DNA methylation exhibit reduced hypocotyl growth at warm temperatures, primarily due to impaired cell elongation. Moreover, DNA hypomethylation compromises auxin biosynthesis and transport in response to warmth, partially by reducing PIF4 protein levels. Notably, the loss of DNA methylation leads to increased expression of SUPPRESSOR OF drm1 drm2 cmt3 (SDC), which in turn restricts hypocotyl elongation during thermomorphogenesis. Finally, we demonstrate that DNAme regulates the inhibition of SDC expression to promote gibberellin biosynthesis. Our findings underscore the critical role of DNA methylation in modulating gene expression in response to temperature fluctuations and provide new insights into the epigenetic regulation of thermomorphogenesis |
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| Description: | Date Revised 10.03.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1460-2431 |
| DOI: | 10.1093/jxb/eraf105 |