Succinic acid synthesis regulated by succinyl-coenzyme A ligase (SUCLA) plays an important role in root response to alkaline salt stress in Leymus chinensis
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 220(2025) vom: 15. März, Seite 109485 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
|
| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Alkaline salt Leymus chinensis Succinic acid Succinyl-coenzyme A ligase (SUCLA) Succinic Acid AB6MNQ6J6L Plant Proteins Coenzyme A Ligases EC 6.2.1.- |
| Zusammenfassung: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. Alkaline salts have more severe adverse effects on plant growth and development than neutral salts do. However, the adaptive mechanisms of plants to alkaline salt stress remain poorly understood, especially at the molecular level. The Songnen Plain in northeast China is composed of typical 'soda' saline-alkali soil, with NaHCO3 and Na2CO3 as the predominant alkaline salts (pH ≥ 9.2). Leymus chinensis can grow on this saline-alkali land, showing strong adaptability. We investigated the role of succinic acid and genes regulating its synthesis in the response to alkaline salt stress in L. chinensis roots. Compared to the neutral salt (NaCl) and high pH treatments, the alkaline salt (NaHCO3 and Na2CO3) treatment specifically caused changes in 11 organic acids, of which the increase in succinic acid was the greatest. The exogenous addition of succinic acid alleviates the damage of alkaline salt to L. chinensis roots. Further, two genes encoding succinyl-coenzyme A ligase (SUCLA) subunits that regulate succinic acid synthesis, LcSUCLAα and LcSUCLAβ, were identified; these genes interact and were localized within mitochondria. Overexpression of LcSUCLAα and LcSUCLAβ caused an increase in succinic acid and enhanced tolerance of NaHCO3 in transgenic rice seedlings. These results suggest that LcSUCLAα and LcSUCLAβ may be involved in the response to alkaline salt stress through the regulation of succinic acid synthesis |
|---|---|
| Beschreibung: | Date Completed 29.04.2025 Date Revised 29.04.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.109485 |