Physcomitrium LATERAL SUPPRESSOR genes promote formative cell divisions to produce germ cell lineages in both male and female gametangia

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 245(2025), 5 vom: 01. März, Seite 2004-2015
1. Verfasser:	Horiuchi, Yuta (VerfasserIn)
Weitere Verfasser:	Umakawa, Naoyuki, Otani, Rina, Tamada, Yosuke, Kosetsu, Ken, Hiwatashi, Yuji, Wakisaka, Rena, Yoshida, Saiko, Murata, Takashi, Hasebe, Mitsuyasu, Ishikawa, Masaki, Kofuji, Rumiko
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2025
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article GRAS Physcomitrium antheridium archegonium formative cell division gametangium Plant Proteins

Beschreibung
Zusammenfassung:	© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation. The evolution of green plants from aquatic to terrestrial environments is thought to have been facilitated by the acquisition of gametangia, specialized multicellular organs housing gametes. Antheridia and archegonia, responsible for producing and protecting sperm and egg cells, undergo formative cell divisions to produce a cell to differentiate into germ cell lineages and the other cell to give rise to surrounding structures. However, the genes governing this process remain unidentified. We isolated genes expressed during gametangia development from previously established gene-trap lines of Physcomitrium patens and characterized their function during gametangia formation. We identified P. patens LATERAL SUPPRESSOR 1 (PpLAS1) from the gene-trap library, encoding a GRAS transcription factor. The double-deletion mutant with its paralog PpLAS2 failed to form inner cells in both gametangia. PpLASs are expressed in cells undergoing formative cell division, and introducing PpLAS1 into the double-deletion mutant successfully rescued the phenotype. These findings underscore the pivotal role of PpLASs in regulating formative cell divisions, ensuring the separation of reproductive cell lineages from surrounding cells in antheridia and archegonia. Furthermore, they suggest a link between PpLASs and the evolutionary origin of male and female gametangia in the common ancestor of land plants
Beschreibung:	Date Completed 29.04.2025 Date Revised 29.04.2025 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.20372