Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR : validation with an NGS-based approach
In 2006, a PCR method was introduced to subtype Blastocystis by Sanger sequencing of an ≈610 bp amplicon of the 18S rRNA gene. This method, known as barcoding-PCR, has become widespread, although the primer pair used can amplify non-Blastocystis sequences, which can result in false positives. Barcod...
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Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 76(2024), 12 vom: 01. Dez., Seite 581-591
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| 1. Verfasser: |
Nieto-Clavijo, Carlos
(VerfasserIn) |
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| Weitere Verfasser: |
Morales, Liliana,
Delgado-Aldana, Andrés,
Hernández, Paula C,
Torres-Molina, Isabel,
Gonzalez-Cuiza, Amanda,
Cortés-Muñoz, Fabián,
Chaparro-Olaya, Jacqueline |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Blastocystis subtyping
ST35
Santin PCR
next-generation sequencing
semi-nested barcode PCR
RNA, Ribosomal, 18S
DNA, Protozoan |
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