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241223s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2024.112363
|2 doi
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|a pubmed24n1645.xml
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|a (DE-627)NLM381961583
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|a (NLM)39710151
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|a (PII)S0168-9452(24)00390-X
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Guo, Jiang-Fan
|e verfasserin
|4 aut
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|a The Arabidopsis heterotrimeric G protein α subunit binds to and inhibits the inward rectifying potassium channel KAT1
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 28.12.2024
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a Copyright © 2024 Elsevier B.V. All rights reserved.
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|a In animal cells, Gα subunit of the heterotrimeric G proteins can bind to both the N-terminal and C-terminal domains of G-protein-activated inwardly rectifying K+ channels (GIRKs) to inhibit their activities. In Arabidopsis guard cells, the Gα subunit GPA1 mediates multiple stimuli-regulated stomatal movements via inhibiting guard cell inward-rectifying K+ (K+in) current, but it remains unclear whether GPA1 directly interacts with and inhibits the activities of K+in channels. Here, we found that GPA1 interacted with the transmembrane domain rather than the intracellular domain of the Shaker family K+in channel KAT1. Two-Electrode Voltage-Clamp experiments in Xenopus oocytes demonstrated that GPA1 significantly inhibited KAT1 channel activity. However, GPA1 could not inhibit the assembly of KAT1 as well as KAT2 as homo- and hetero-tetramers and alter the subcellular localization and protein stability of these channels. In conclusion, these findings reveal a novel regulatory mechanism for Gα inhibition of the Shaker family K+in channel KAT1 via binding to its channel transmembrane domains but without affecting its subcellular localization, protein stability and the formation of functional homo- and hetero-tetramers. This suggests that in both animal and plant cells, Gα can regulate K+in channels through physical interaction, albeit with differing mechanisms of interaction and regulation
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|a Journal Article
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|a Gα subunit
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|a heterotrimer G proteins
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|a interaction
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|a inward-rectifying K(+) channels
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|a potassium channel in Arabidopsis thaliana 1
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|a transmembrane domains
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|a Zhou, Hui
|e verfasserin
|4 aut
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|a Hu, Zhuo-Ran
|e verfasserin
|4 aut
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1 |
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|a Yang, Ya-Lan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Wen-Bin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Yan-Ru
|e verfasserin
|4 aut
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|a Li, Xue
|e verfasserin
|4 aut
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|a Mulati, Nuerkaimaier
|e verfasserin
|4 aut
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| 700 |
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|a Li, Ying-Xin
|e verfasserin
|4 aut
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|a Wu, Lu
|e verfasserin
|4 aut
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1 |
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|a Long, Yu
|e verfasserin
|4 aut
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| 700 |
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|a He, Jun-Min
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 352(2024) vom: 20. Dez., Seite 112363
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:352
|g year:2024
|g day:20
|g month:12
|g pages:112363
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|u http://dx.doi.org/10.1016/j.plantsci.2024.112363
|3 Volltext
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