The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure
© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.
| Publié dans: | Journal of experimental botany. - 1985. - (2024) vom: 19. Dez. |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2024
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| Accès à la collection: | Journal of experimental botany |
| Sujets: | Journal Article ABA signaling CO2 signaling H2O2 Maize stomata aquaporin stomatal movement |
| Résumé: | © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. The plasma membrane aquaporin ZmPIP1;6 is expressed in maize stomatal complexes, with higher expression during the day than at night. To elucidate the role of ZmPIP1;6 in gas exchange and stomatal movement, it was expressed in maize (inbred line B104) under the control of p35S promoter (OE) or its native promoter fused with mYFP cDNA (mYFP-ZmPIP1;6). In stomatal complexes of the leaf mature zone, mYFP-ZmPIP1;6 showed higher expression in subsidiary cells than in guard cells, with light and dark treatments influencing its subcellular localization. Notably, ZmPIP1;6 internalization increased in dark conditions versus light. Stomatal opening was greater in ZmPIP1;6 OE than in wild type (WT), while closure exhibited greater sensitivity to elevated CO2 concentration or ABA treatment. Our finding revealed that reactive oxygen species (H2O2) was involved in ABA-induced stomatal closure, while ZmPIP1;6 was unable to facilitate H2O2 diffusion when expressed in yeast. Finally, ZmPIP1;6 OE and mYFP-ZmPIP1;6 transgenic plants exhibited higher abaxial stomatal density than WT. Overall, these results indicate that ZmPIP1;6 plays important roles in stomatal opening and CO2- and ABA-induced stomatal closure |
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| Description: | Date Revised 19.12.2024 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1460-2431 |
| DOI: | 10.1093/jxb/erae500 |