The homeodomain leucine zipper protein RhHB22 promotes petal senescence by repressing ascorbic acid biosynthesis in rose

© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink serv...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 76(2025), 6 vom: 09. Apr., Seite 1704-1717
1. Verfasser: Li, Ping (VerfasserIn)
Weitere Verfasser: Qiu, Yuexuan, Wang, Rui, Zhang, Bingjie, Ma, Yanxing, Sun, Xiaoming, Gao, Junping, Jiang, Yunhe
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article GGP1 Ascorbic acid HD-ZIP ethylene petal senescence reactive oxygen species rose (Rosa hybrida) Ascorbic Acid PQ6CK8PD0R mehr... Plant Proteins Ethylenes 91GW059KN7 Transcription Factors Homeodomain Proteins Reactive Oxygen Species
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520 |a © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. 
520 |a Premature petal senescence dramatically reduces flower quality and value. Ethylene and reactive oxygen species (ROS) are key players in accelerating rose petal senescence, but the molecular mechanism by which ethylene antagonizes ROS scavenging is not well understood. Here, we showed that ethylene reduces ascorbic acid (AsA) production, leading to the accumulation of ROS and hastening petal senescence. Ethylene treatment suppressed the expression of GDP-l-galactose phosphorylase 1 (RhGGP1), encoding the rate-controlling enzyme in AsA biosynthesis. A HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP) II transcription factor, RhHB22, directly bound to the promoter of RhGGP1 and inhibited its transcription. RhHB22 is induced by ethylene, and silencing of RhHB22 increased RhGGP1 expression and AsA production, resulting in reduced H2O2 accumulation and delayed petal senescence. Additionally, the delayed petal senescence symptoms of RhHB22-silenced plants were suppressed by silencing RhGGP1. Moreover, the expression of RhGGP1, which is suppressed by ethylene in wild-type petals, was significantly compromised in RhHB22-silenced petals. These findings uncover the transcriptional regulatory mechanism by which ethylene promotes ROS accumulation and petal senescence by inhibiting AsA biosynthesis, enhance our understanding of ethylene-induced petal senescence, and provide novel insights for improving the longevity of cut flowers 
650 4 |a Journal Article 
650 4 |a GGP1 
650 4 |a Ascorbic acid 
650 4 |a HD-ZIP 
650 4 |a ethylene 
650 4 |a petal senescence 
650 4 |a reactive oxygen species 
650 4 |a rose (Rosa hybrida) 
650 7 |a Ascorbic Acid  |2 NLM 
650 7 |a PQ6CK8PD0R  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Ethylenes  |2 NLM 
650 7 |a ethylene  |2 NLM 
650 7 |a 91GW059KN7  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a Homeodomain Proteins  |2 NLM 
650 7 |a Reactive Oxygen Species  |2 NLM 
700 1 |a Qiu, Yuexuan  |e verfasserin  |4 aut 
700 1 |a Wang, Rui  |e verfasserin  |4 aut 
700 1 |a Zhang, Bingjie  |e verfasserin  |4 aut 
700 1 |a Ma, Yanxing  |e verfasserin  |4 aut 
700 1 |a Sun, Xiaoming  |e verfasserin  |4 aut 
700 1 |a Gao, Junping  |e verfasserin  |4 aut 
700 1 |a Jiang, Yunhe  |e verfasserin  |4 aut 
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773 1 8 |g volume:76  |g year:2025  |g number:6  |g day:09  |g month:04  |g pages:1704-1717 
856 4 0 |u http://dx.doi.org/10.1093/jxb/erae503  |3 Volltext 
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