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|a 10.1016/j.plantsci.2024.112357
|2 doi
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|a pubmed24n1637.xml
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|a (DE-627)NLM381614697
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|a (NLM)39675386
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|a (PII)S0168-9452(24)00384-4
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|a DE-627
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|a eng
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|a Li, Jie
|e verfasserin
|4 aut
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|a Effect analysis of S5-interacting genes on rice hybrid sterility using nontransgenic gamete killer
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|c 2024
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 19.12.2024
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a Copyright © 2024 Elsevier B.V. All rights reserved.
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|a While hybrids between japonica and indica rice exhibit strong heterosis, they often suffer from hybrid sterility (HS). Hybrid fertility of the embryo sac is predominantly regulated by a three-gene system (comprising closely linked ORF3, ORF4 and ORF5) at rice S5 locus. The cooperation of ORF5+ and ORF4+ can result in endoplasmic reticulum (ER) stress and sporophytically kill all embryo sacs, while ORF3+ can gametophytically protect the residing embryo sac. We previously identified four S5-interacting genes (SIGs) using a transgenic line BLORF5+ (Balilla carrying transgenic ORF5+) and a wide compatibility variety Dular (DL or D). Homozygote and hemizygote of ORF5+ transgene had significantly different spikelet fertility (SF), which disturbed the phenotypic effects of SIGs. However, HS effects of SIGs under the endogenous (nontransgenic) gamete killer remained unknown. We formerly constructed a semisterile near isogenic line (NIL) S5-BL/NJ by introgressing S5 fragment of indica rice Nanjing11 (NJ or N, carrying ORF3+ORF4-ORF5+ haplotypes) into the genome of japonica rice Balilla (BL or B, carrying ORF3-ORF4+ORF5- haplotypes). The gamete-protecting effect of ORF3+ in NJ may confuse SF effect of the SIGs, so we knocked out ORF3+ of S5-NJ/NJ and crossed it with BL to get gamete-killing S5-BL/NJΔORF3+, which can kill all (KA) gametes (abbreviated as enS5KA). Compared with the exS5KA line (a NIL carrying ORF5+ transgenic, wihch can kill all gamete), the enS5KA line conferred SIGs a more pronounced SF effect. The enS5KA,SIG-DDDD (four SIGs carry homozygous DL alleles) genotype caused a SF of about 78 %, while SF of exS5KA,SIG-DDDD was only about 62 %. Moreover, all SIGs acted in a sporophytic manner without segregation distortion of genotype. Although enS5KA,SIG-DDDD plants had high SF, the ER stress still existed. The ovule section revealed that enS5KA,SIG-BBBB genotype (four SIGs carry homozygous BL allele, with ER stress and SF < 5 %) caused abnormal degradation of nucellar cells and functional megaspores. In contrast, enS5KA,SIG-DDDD genotype preserved most nucellar cells and functional megaspores. These results lay the foundation for further research on HS mechanism of S5 and SIGs and cloning of candidate genes
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|a Journal Article
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|a Endoplasmic reticulum stress
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| 650 |
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|a Hybrid sterility
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| 650 |
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|a Reproductive isolation
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|a Segregation distortion
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|a Speciation
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|a Huang, Fu
|e verfasserin
|4 aut
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| 700 |
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|a Jiang, Yingxia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rao, Jianglei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fan, Yourong
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Jiangyi
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 352(2024) vom: 13. Dez., Seite 112357
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:352
|g year:2024
|g day:13
|g month:12
|g pages:112357
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|u http://dx.doi.org/10.1016/j.plantsci.2024.112357
|3 Volltext
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