Impact of chemical oxygen demand to nitrogen ratio on ANAMMOX bacterial growth in an up-flow anaerobic sludge blanket reactor
© 2024 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY-NC 4.0), which permits copying, adaptation and redistribution for non-commercial purposes, provided the original work is properly cited (http://creativecommons.org/license...
| Veröffentlicht in: | Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 90(2024), 11 vom: 14. Dez., Seite 2978-2990 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
|
| Zugriff auf das übergeordnete Werk: | Water science and technology : a journal of the International Association on Water Pollution Research |
| Schlagworte: | Journal Article ANAMMOX performance C/N ratio denitrification gene expression microbial community nitrogen removal Nitrogen N762921K75 Sewage mehr... |
| Zusammenfassung: | © 2024 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY-NC 4.0), which permits copying, adaptation and redistribution for non-commercial purposes, provided the original work is properly cited (http://creativecommons.org/licenses/by-nc/4.0/). While several studies have investigated the effect of varying carbon-to-nitrogen (C/N) ratios on the ANAMMOX performance, there is still a research gap in illustrating the shift in 16S rRNA gene copy number and functional microbial population during operation. Hence, this study focuses on utilizing a reference gene and target functional genes to demonstrate the synergetic interaction between ANAMMOX, ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB), using an up-flow anaerobic sludge blanket (UASB) under different C/N conditions. It was demonstrated that elevating the C/N ratio from 1.0 to 2.0 reduced the COD and NH4+-N removal efficiencies from 80.12 to 48.62% and from 88.99 to 72.59%, respectively. Based on the qPCR evaluation, at the C/N ratio of 1.5, the abundance of ANAMMOX, AOB, Nitrobacter, and Nitrospira was 2.52 × 106, 82, 5.39 × 103, and 12.98 × 103 copies/μL, respectively. However, with the further increase of C/N ratio to 2.0, their abundance was reduced to 1.09 × 106, 46, 0.98 × 103, and 3.47 × 103 copies/μL, respectively. The expression of hzo gene encoding for hydrazine dehydrogenase was 169-folds at C/N = 1 and almost inhibited at C/N = 2. The results of microbial population structure using 16S rRNA reverse transcriptase (RT)-qPCR technique depicted a competition between ANAMMOX and heterotrophic bacteria for the available substrate at higher C/N ratios |
|---|---|
| Beschreibung: | Date Completed 14.12.2024 Date Revised 14.12.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 0273-1223 |
| DOI: | 10.2166/wst.2024.376 |