Triticain alpha represents the major active papain-like cysteine protease in naturally senescing and ozone-treated leaves of wheat

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 219(2024) vom: 04. Dez., Seite 109380
1. Verfasser:	Havé, Marien (VerfasserIn)
Weitere Verfasser:	Espinasse, Christophe, Cottyn-Boitte, Betty, Puga-Freitas, Ruben, Bagard, Matthieu, Balliau, Thierry, Zivy, Michel, Ganeshan, Seedhabadee, Chibbar, Ravindra N, Castell, Jean-François, Bethenod, Olivier, Leitao, Luis, Repellin, Anne
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Activity-based protein profiling Flag leaf senescence Papain-like cysteine protease Triticain alpha Tropospheric ozone Wheat (Triticum aestivum L.)

Beschreibung
Zusammenfassung:	Copyright © 2024 Elsevier Masson SAS. All rights reserved. Current background tropospheric ozone (O3) concentrations have significant adverse effects on wheat. O3 generally induces oxidative damages and premature leaf senescence leading to important yield losses. As leaf protein degradation and recycling is involved in both maintaining cell longevity during abiotic stresses and performing efficient nitrogen remobilization during senescence, we aimed to identify proteases involved in acidic endoproteolytic activities during natural and O3-induced leaf senescence in wheat. Field-grown plants of two winter wheat cultivars were exposed to ambient and semi-controlled chronic O3 concentrations, from pre-anthesis to grain harvest. Yield parameters were impacted by the most elevated O3 exposure for both cultivars. At the cellular level, our analysis revealed that both natural leaf senescence and O3 treatments induced a stimulation of acidic (pH 5.5) endoproteolytic activities, mostly due to papain-like cysteine proteases (PLCPs). Identification of active PLCPs using activity-based protein profiling (ABPP) revealed that Triticain α was the major active PLCP in senescing flag leaves and the only PLCP whose abundance increased with O3 stress, a result of positive transcriptional regulation. Our study provides novel insight into the implication of PLCP-mediated proteolysis in O3 sensitivity in a major crop
Beschreibung:	Date Revised 09.12.2024 published: Print-Electronic Citation Status Publisher
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2024.109380