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241209s2024 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202413957
|2 doi
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|a pubmed25n1270.xml
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Yue
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Clickable, Thermally Responsive Hydrogels Enabled by Recombinant Spider Silk Protein and Spy Chemistry for Sustained Neurotrophin Delivery
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|a Date Revised 09.12.2024
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a © 2024 Wiley‐VCH GmbH.
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|a The ability to deliver protein therapeutics in a minimally invasive, safe, and sustained manner, without resorting to viral delivery systems, will be crucial for treating a wide range of chronic injuries and diseases. Among these challenges, achieving axon regeneration and functional recovery post-injury or disease in the central nervous system remains elusive to most clinical interventions, constantly calling for innovative solutions. Here, a thermally responsive hydrogel system utilizing recombinant spider silk protein (spidroin) is developed. The protein solution undergoes rapid sol-gel transition at an elevated temperature (37 °C) following brief sonication. This thermally triggered gelation confers injectability to the system. Leveraging SpyTag/SpyCatcher chemistry, the hydrogel, composed of SpyTag-fusion spidroin, can be functionalized with diverse SpyCatcher-fusion bioactive motifs, such as neurotrophic factors (e.g., ciliary neurotrophic factor) and cell-binding ligands (e.g., laminin), rendering it well-suited for neuronal culturing. More importantly, the intravitreous injection of the protein materials decorated with SpyCatcher-fusion CNTF into the vitreous body after optic nerve injury leads to prolonged JAK/STAT3 signaling, increased neuronal survival, and enhanced axon regeneration. This study illustrates a generalizable material system for injectable and sustained delivery of protein therapeutics for neuroprotection and regeneration, with the potential for extension to other chronic diseases and injuries
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|a Journal Article
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|a axon regeneration
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| 650 |
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4 |
|a injectable hydrogel
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| 650 |
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|a neuroprotection
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| 650 |
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4 |
|a protein delivery
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| 650 |
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4 |
|a spider silk protein
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| 700 |
1 |
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|a Yang, Chao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Shiyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Yiren
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Manjia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Zewei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Xin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Duan, Liting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Kai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Fei
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g (2024) vom: 08. Dez., Seite e2413957
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnas
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| 773 |
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|g year:2024
|g day:08
|g month:12
|g pages:e2413957
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|u http://dx.doi.org/10.1002/adma.202413957
|3 Volltext
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