A barley MLA immune receptor is activated by a fungal nonribosomal peptide effector for disease susceptibility

A barley MLA immune receptor is activated by a fungal nonribosomal peptide effector for disease susceptibility

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - (2024) vom: 06. Dez.
1. Verfasser: Leng, Yueqiang (VerfasserIn)
Weitere Verfasser: Kümmel, Florian, Zhao, Mingxia, Molnár, István, Doležel, Jaroslav, Logemann, Elke, Köchner, Petra, Xi, Pinggen, Yang, Shengming, Moscou, Matthew J, Fiedler, Jason D, Du, Yang, Steuernagel, Burkhard, Meinhardt, Steven, Steffenson, Brian J, Schulze-Lefert, Paul, Zhong, Shaobin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Bipolaris sorokiniana cell death innate immunity necrotrophic pathogen nucleotide‐binding leucine‐rich‐repeat receptors
Beschreibung
Zusammenfassung:© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
The barley Mla locus contains functionally diversified genes that encode intracellular nucleotide-binding leucine-rich repeat receptors (NLRs) and confer strain-specific immunity to biotrophic and hemibiotrophic fungal pathogens. In this study, we isolated a barley gene Scs6, which is an allelic variant of Mla genes but confers susceptibility to the isolate ND90Pr (BsND90Pr) of the necrotrophic fungus Bipolaris sorokiniana. We generated Scs6 transgenic barley lines and showed that Scs6 is sufficient to confer susceptibility to BsND90Pr in barley genotypes naturally lacking the receptor. The Scs6-encoded NLR (SCS6) is activated by a nonribosomal peptide (NRP) effector produced by BsND90Pr to induce cell death in barley and Nicotiana benthamiana. Domain swaps between MLAs and SCS6 reveal that the SCS6 leucine-rich repeat domain is a specificity determinant for receptor activation by the NRP effector. Scs6 is maintained in both wild and domesticated barley populations. Our phylogenetic analysis suggests that Scs6 is a Hordeum-specific innovation. We infer that SCS6 is a bona fide immune receptor that is likely directly activated by the nonribosomal peptide effector of BsND90Pr for disease susceptibility in barley. Our study provides a stepping stone for the future development of synthetic NLR receptors in crops that are less vulnerable to modification by necrotrophic pathogens
Beschreibung:Date Revised 06.12.2024
published: Print-Electronic
Citation Status Publisher
ISSN:1469-8137
DOI:10.1111/nph.20289