DcWRKY15 positively regulates anthocyanin biosynthesis during petal coloration in Dianthus caryophyllus

DcWRKY15 positively regulates anthocyanin biosynthesis during petal coloration in Dianthus caryophyllus

Copyright © 2024 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 219(2024) vom: 28. Nov., Seite 109358
1. Verfasser: Fu, Xueqing (VerfasserIn)
Weitere Verfasser: Li, Shu, Zhang, Yaojie, Zheng, Han, Liu, Hang, Liu, Pin, Li, Ling, Zhao, Jingya, Gao, Yanhua, Tang, Dongqin, Wang, Yuliang, Tang, Kexuan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Anthocyanin Dianthus caryophyllus Pigmentation Regulation WRKY
Beschreibung
Zusammenfassung:Copyright © 2024 Elsevier Masson SAS. All rights reserved.
Petal pigmentation in carnations is closely associated with the biosynthesis of anthocyanins. This biosynthetic process is tightly regulated by transcription factors, which activate or repress key genes involved in anthocyanin production. Here, we aim to explore the mechanisms involved in the transcriptional regulation of anthocyanin biosynthesis in carnation petals. We identified DcWRKY15 as a critical regulator influencing anthocyanin production in these petals. DcWRKY15 expression showed a strong correlation with the expression levels of genes associated with anthocyanin biosynthesis, peaking during early petal development stages. The findings from the dual-LUC, VIGS, Y1H and EMSA assays demonstrated that DcWRKY15 played a positive regulatory role in anthocyanin biosynthesis. DcWRKY15 achieved this by binding directly to the promoters of DcCHS and DcF3H, thereby enhancing their expression. Additionally, DcWRKY15 interacts with the repressor DcMYB2, which reduces its capacity to enhance anthocyanin biosynthesis, particularly during the later stages of petal development. These findings offer new insights into the molecular mechanisms responsible for petal coloration in carnations, highlighting the complex interplay between activator and repressor transcription factors
Beschreibung:Date Revised 01.12.2024
published: Print-Electronic
Citation Status Publisher
ISSN:1873-2690
DOI:10.1016/j.plaphy.2024.109358