Characterization of pecan PEBP family genes and the potential regulation role of CiPEBP-like1 in fatty acid synthesis
Copyright © 2024 Elsevier B.V. All rights reserved.
| Publié dans: | Plant science : an international journal of experimental plant biology. - 1985. - 351(2025) vom: 22. Feb., Seite 112326 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Plant science : an international journal of experimental plant biology |
| Sujets: | Journal Article CiPEBP-like1 PEBP family fatty acid overexpression pecan Plant Proteins Fatty Acids Phosphatidylethanolamine Binding Protein |
| Résumé: | Copyright © 2024 Elsevier B.V. All rights reserved. Phosphatidyl ethanolamine-binding protein (PEBP) plays important roles in plant growth and development. However, few studies have investigated the PEBP gene family in pecan (Carya illinoinensis), particularly the function of the PEBP-like subfamily. In this study, we identified 12 PEBP genes from the pecan genome and classified them into four subfamilies: MFT-like, FT-like, TFL1-like and PEBP-like. Multiple sequence alignment, gene structure, and conserved motif analyses indicated that pecan PEBP subfamily genes were highly conserved. Cis-element analysis revealed that many light responsive elements and plant hormone-responsive elements are found in CiPEBPs promoters. Additionally, RNA-seq and RT-qPCR showed that CiPEBP-like1 was highly expressed during kernel filling stage. GO and KEGG enrichment analysis further indicated that CiPEBP-like1 was involved in fatty acid biosynthesis and metabolism progress. Overexpression of CiPEBP-like1 led to earlier flowering and altered fatty acid composition in Arabidopsis seeds. RT-qPCR confirmed that CiPEBP-like1 promoted fatty acid synthesis by regulating the expression of key genes. Overall, this study contributes to a comprehensive understanding of the potential functions of the PEBP family genes and lay a foundation to modifying fatty acid composition in pecan kernel |
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| Description: | Date Completed 07.12.2024 Date Revised 07.12.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2024.112326 |