Plant PI4P is required for bacteria to translocate type-3 effectors

Plant PI4P is required for bacteria to translocate type-3 effectors

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 245(2025), 2 vom: 20. Jan., Seite 748-766
1. Verfasser: Peng, Jinfeng (VerfasserIn)
Weitere Verfasser: Zhang, Liyuan, Lu, Kai, Chen, Xiaochen, Pang, Hao, Yao, Xiaohui, Li, Ping, Cao, Peng, Li, Xiaoxu, Wang, Zuodong, Qin, Lina, Zhou, Miao, Wang, Maoling, Li, Qizhen, Qiu, Chunyu, Sun, Mingxin, Li, Yufen, Gong, Liping, Wei, Xinlin, Wang, Siyi, Chen, Jiajia, Lu, Chongchong, Zou, Shenshen, Ding, Xinhua, Chen, Lei, Zhang, Meixiang, Dong, Hansong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article HrpF phosphatidylinositol 4‐phosphate translocase translocation type‐3 effector type‐3 translocator Phosphatidylinositol Phosphates phosphatidylinositol 4-phosphate Bacterial Proteins
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520 |a Type-3 effectors (T3E) of phytopathogenic Gram-negative bacteria fulfill a virulent role, causing disease, or an avirulent role, inducing immunity, following their translocation into plant cells. This study aimed to validate the hypothesis that bacterial T3E translocation requires lipidic compounds in plant cell membranes. Based on genetic, molecular, and biochemical assays, we determined that phosphatidylinositol 4-phosphate (PI4P) associated with plant cell membranes is essential for the translocation of T3E by bacterial pathogens. Replicate experimental data revealed that PI4P cooperates with the type-3 translocase HrpF to facilitate the translocation of effectors TAL and Xop from Xanthomonas oryzae and Hop from Pseudomonas syringae into the cells of Oryza sativa and Nicotiana benthamiana, respectively. Genetic and molecular analyses confirmed that, once translocated into plant cells, the distinct effectors induce disease or immunity. Combined genetic and pharmacological analyses revealed that when PI4P content is suppressed via genetic or pharmacological measures, the T3 effector translocation is considerably suppressed, resulting in serious inhibition of bacterial infection. Overall, these findings demonstrate that cooperative functioning of HrpF-PI4P is conserved in bacterial effectors and plants 
650 4 |a Journal Article 
650 4 |a HrpF 
650 4 |a phosphatidylinositol 4‐phosphate 
650 4 |a translocase 
650 4 |a translocation 
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650 4 |a type‐3 translocator 
650 7 |a Phosphatidylinositol Phosphates  |2 NLM 
650 7 |a phosphatidylinositol 4-phosphate  |2 NLM 
650 7 |a Bacterial Proteins  |2 NLM 
700 1 |a Zhang, Liyuan  |e verfasserin  |4 aut 
700 1 |a Lu, Kai  |e verfasserin  |4 aut 
700 1 |a Chen, Xiaochen  |e verfasserin  |4 aut 
700 1 |a Pang, Hao  |e verfasserin  |4 aut 
700 1 |a Yao, Xiaohui  |e verfasserin  |4 aut 
700 1 |a Li, Ping  |e verfasserin  |4 aut 
700 1 |a Cao, Peng  |e verfasserin  |4 aut 
700 1 |a Li, Xiaoxu  |e verfasserin  |4 aut 
700 1 |a Wang, Zuodong  |e verfasserin  |4 aut 
700 1 |a Qin, Lina  |e verfasserin  |4 aut 
700 1 |a Zhou, Miao  |e verfasserin  |4 aut 
700 1 |a Wang, Maoling  |e verfasserin  |4 aut 
700 1 |a Li, Qizhen  |e verfasserin  |4 aut 
700 1 |a Qiu, Chunyu  |e verfasserin  |4 aut 
700 1 |a Sun, Mingxin  |e verfasserin  |4 aut 
700 1 |a Li, Yufen  |e verfasserin  |4 aut 
700 1 |a Gong, Liping  |e verfasserin  |4 aut 
700 1 |a Wei, Xinlin  |e verfasserin  |4 aut 
700 1 |a Wang, Siyi  |e verfasserin  |4 aut 
700 1 |a Chen, Jiajia  |e verfasserin  |4 aut 
700 1 |a Lu, Chongchong  |e verfasserin  |4 aut 
700 1 |a Zou, Shenshen  |e verfasserin  |4 aut 
700 1 |a Ding, Xinhua  |e verfasserin  |4 aut 
700 1 |a Chen, Lei  |e verfasserin  |4 aut 
700 1 |a Zhang, Meixiang  |e verfasserin  |4 aut 
700 1 |a Dong, Hansong  |e verfasserin  |4 aut 
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