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241116s2025 xx |||||o 00| ||eng c |
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|a 10.1111/nph.20271
|2 doi
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|a pubmed24n1651.xml
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|a (NLM)39545639
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Ramakers, Lennart A I
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Unravelling the different components of nonphotochemical quenching using a novel analytical pipeline
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|c 2025
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 19.12.2024
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|a Date Revised 04.01.2025
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
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|a Photoprotection in plants includes processes collectively known as nonphotochemical quenching (NPQ), which quench excess excitation-energy in photosystem II. NPQ is triggered by acidification of the thylakoid lumen, which leads to PsbS-protein protonation and violaxanthin de-epoxidase activation, resulting in zeaxanthin accumulation. Despite extensive study, questions persist about the mechanisms of NPQ. We have set up a novel analytical pipeline to disentangle NPQ induction curves measured at many light intensities into a limited number of different kinetic components. To validate the method, we applied it to Chl-fluorescence measurements, which utilised the saturating-pulse methodology, on wild-type (wt) and zeaxanthin-lacking (npq1) Arabidopsis thaliana plants. NPQ induction curves in wt and npq1 can be explained by four components ( α , β , γ and δ ). The fastest two ( β and γ ) correlate with pH difference formed across the thylakoid membrane in wt and npq1. In wt, the slower component ( α ) appears to be due to the formation of zeaxanthin-related quenching whilst for npq1, this component is 'replaced' by a slower component ( δ ), which reflects a photoinhibition-like process that appears in the absence of zeaxanthin-induced quenching. Expanding this approach will allow the effects of mutations and other abiotic-stress factors to be directly probed by changes in these underlying components
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|a Journal Article
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|a Arabidopsis thaliana
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|a PsbS
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|a multivariate analysis
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| 650 |
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|a nonphotochemical quenching
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| 650 |
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4 |
|a xanthophyll cycle
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| 650 |
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4 |
|a zeaxanthin
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|a Zeaxanthins
|2 NLM
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| 650 |
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|a Photosystem II Protein Complex
|2 NLM
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| 650 |
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|a Chlorophyll
|2 NLM
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| 650 |
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7 |
|a 1406-65-1
|2 NLM
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| 650 |
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7 |
|a Xanthophylls
|2 NLM
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| 700 |
1 |
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|a Harbinson, Jeremy
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wientjes, Emilie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a van Amerongen, Herbert
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 245(2025), 2 vom: 30. Jan., Seite 625-636
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:245
|g year:2025
|g number:2
|g day:30
|g month:01
|g pages:625-636
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|u http://dx.doi.org/10.1111/nph.20271
|3 Volltext
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