Discovery and functional characterization of two novel glycosyltransferases associated with the biosynthesis of α-hederin in Dipsacus asperoides
Copyright © 2024 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 217(2024) vom: 15. Dez., Seite 109273 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2024
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Biosynthesis Glycosyltransferases Transcriptome Triterpenoid saponin α-hederin EC 2.4.- Oleanolic Acid 6SMK8R7TGJ Saponins plus... |
| Résumé: | Copyright © 2024 Elsevier Masson SAS. All rights reserved. Triterpenoid saponins are crucial natural products widely distributed in various medicinal plants, with Dipsacus asperoides being particularly rich in these compounds. However, the glycosyltransferases responsible for the biosynthesis of α-hederin, one of the primary bioactive secondary metabolites in D. asperoides, have not been elucidated. In this study, transcriptomic and compound analyses revealed 359 differentially expressed genes associated with secondary metabolism, with 271 involved in triterpenoid saponin glycosylation. Through correlation analysis, 71 candidate glycosyltransferases were identified, and two novel glycosyltransferases were functionally characterized. It was shown that DaUGT121 catalyzes the conversion of hederagenin into cauloside A, while DaUGT103 acts as a cauloside A 1,2-rhamnosyltransferase transforming cauloside A into α-hederin. These findings illuminate the biosynthesis of triterpenoid saponins in D. asperoides, providing insights into the molecular mechanisms and offering novel tools for synthesizing natural products with diverse sugar moieties |
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| Description: | Date Completed 02.12.2024 Date Revised 02.12.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2024.109273 |