Asymmetric genome merging leads to gene expression novelty through nucleo-cytoplasmic disruptions and transcriptomic shock in Chlamydomonas triploids

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 245(2025), 2 vom: 08. Jan., Seite 869-884
1. Verfasser:	Prost-Boxoen, Lucas (VerfasserIn)
Weitere Verfasser:	Bafort, Quinten, Van de Vloet, Antoine, Almeida-Silva, Fabricio, Paing, Yunn Thet, Casteleyn, Griet, D'hondt, Sofie, De Clerck, Olivier, Van de Peer, Yves
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2025
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Chlamydomonas reinhardtii RNA‐Seq allopolyploidy experimental evolution laboratory natural selection (LNS) genome merging transcriptomics

Beschreibung
Zusammenfassung:	© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation. Genome merging is a common phenomenon causing a wide range of consequences on phenotype, adaptation, and gene expression, yet its broader implications are not well-understood. Two consequences of genome merging on gene expression remain particularly poorly understood: dosage effects and evolution of expression. We employed Chlamydomonas reinhardtii as a model to investigate the effects of asymmetric genome merging by crossing a diploid with a haploid strain to create a novel triploid line. Five independent clonal lineages derived from this triploid line were evolved for 425 asexual generations in a laboratory natural selection experiment. Utilizing fitness assays, flow cytometry, and RNA-Seq, we assessed the immediate consequences of genome merging and subsequent evolution. Our findings reveal substantial alterations in genome size, gene expression, protein homeostasis, and cytonuclear stoichiometry. Gene expression exhibited expression-level dominance and transgressivity (i.e. expression level higher or lower than either parent). Ongoing expression-level dominance and a pattern of 'functional dominance' from the haploid parent was observed. Despite major genomic and nucleo-cytoplasmic disruptions, enhanced fitness was detected in the triploid strain. By comparing gene expression across generations, our results indicate that proteostasis restoration is a critical component of rapid adaptation following genome merging in Chlamydomonas reinhardtii and possibly other systems
Beschreibung:	Date Completed 18.12.2024 Date Revised 09.05.2025 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.20249