Identification of genes associated with sex expression and sex determination in hemp (Cannabis sativa L.)

© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 76(2025), 1 vom: 01. Jan., Seite 175-190
1. Verfasser: Shi, Jiaqi (VerfasserIn)
Weitere Verfasser: Toscani, Matteo, Dowling, Caroline A, Schilling, Susanne, Melzer, Rainer
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Cannabis sativa REM TM8 bZIP differential gene expression dioecy hemp sex determination transcriptomics mehr... weighted gene co-expression network analysis Plant Proteins
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520 |a Dioecy in flowering plants has evolved independently many times, and thus the genetic mechanisms underlying sex determination are diverse. In hemp (Cannabis sativa), sex is controlled by a pair of sex chromosomes (XX for females and XY for males). In an attempt to understand the molecular mechanism responsible for sex expression in hemp plants, we carried out RNA sequencing of male and female plants at different developmental stages. Using a pipeline involving differential gene expression analysis and weighted gene co-expression network analysis, we identified genes important for male and female flower development. We also demonstrate that sex-biased expression is already established at very early vegetative stages, before the onset of reproductive development, and identify several genes encoding transcription factors of the REM, bZIP, and MADS families as candidate sex-determination genes in hemp. Our findings demonstrate that the gene regulatory networks governing male and female development in hemp diverge at a very early stage, leading to profound morphological differences between male and female hemp plants 
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650 4 |a weighted gene co-expression network analysis 
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700 1 |a Toscani, Matteo  |e verfasserin  |4 aut 
700 1 |a Dowling, Caroline A  |e verfasserin  |4 aut 
700 1 |a Schilling, Susanne  |e verfasserin  |4 aut 
700 1 |a Melzer, Rainer  |e verfasserin  |4 aut 
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