Nanoplastics Immune Impact, Detection, and Internalization after Human Blood Exposure by Single-Cell Mass Cytometry

Nanoplastics : Immune Impact, Detection, and Internalization after Human Blood Exposure by Single-Cell Mass Cytometry

© 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - (2024) vom: 24. Okt., Seite e2413413
1. Verfasser: Fusco, Laura (VerfasserIn)
Weitere Verfasser: Gazzi, Arianna, Giro, Linda, Schefer, Roman B, D'Almeida, Sènan Mickael, Cagliani, Roberta, Zoccheddu, Martina, Uyar, Recep, Besbinar, Ömur, Çelik, Doğantan, Yilmazer, Acelya, Mitrano, Denise M, Orecchioni, Marco, Delogu, Lucia Gemma
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article CyTOF biocompatibility blood exposure immune cells pollution safety
Beschreibung
Zusammenfassung:© 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.
The increasing exposure to nanoplastics (NPs) raises significant concerns for human health, primarily due to their potential bioaccumulative properties. While NPs have recently been detected in human blood, their interactions with specific immune cell subtypes and their impact on immune regulation remain unclear. In this proof-of-concept study, model palladium-doped polystyrene NPs (PS-Pd NPs) are utilized to enable single-cell mass cytometry (CyTOF) detection. The size-dependent impact of carboxylate polystyrene NPs (50-200 nm) is investigated across 15 primary immune cell subpopulations using CyTOF. By taking advantage of Pd-doping for detecting PS-Pd NPs, this work evaluates their impact on human immune-cells at the single-cell level following blood exposure. This work traces PS-Pd NPs in 37 primary immune-cell subpopulations from human blood, quantifying the palladium atom count per cell by CyTOF while simultaneously assessing the impact of PS-Pd NPs on cell viability, functionality, and uptake. These results demonstrate that NPs can interact with, interfere with, and translocate into several immune cell subpopulations after exposure. In vivo distribution experiments in mice further confirmed their accumulation in immune cells within the liver, blood, and spleen, particularly in monocytes, macrophages, and dendritic cells. These findings provide valuable insights into the impact of NPs on human health
Beschreibung:Date Revised 25.10.2024
published: Print-Electronic
Citation Status Publisher
ISSN:1521-4095
DOI:10.1002/adma.202413413