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241021s2024 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.4c03104
|2 doi
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|a pubmed24n1592.xml
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|a (DE-627)NLM379158841
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|a (NLM)39429033
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Hanashima, Shinya
|e verfasserin
|4 aut
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| 245 |
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|a Lipid Compositions of Liquid-Ordered and Liquid-Disordered Phases in Ternary Membranes of Sphingomyelin, Cholesterol, and Dioleoylphosphatidylcholine Determined by 2H NMR
|b Stearoyl-Sphingomyelin Compared with Its Palmitoyl Counterpart
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 29.10.2024
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|a Date Revised 05.11.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Sphingomyelin (SM) and cholesterol are the major lipids in the signaling platforms of cell membranes, known as lipid rafts. In particular, SM with a stearoyl chain (C18-SM) is abundant in specific tissues such as the brain, the most cholesterol-rich organ, whereas the distribution of palmitoyl (C16)-SM is ubiquitous. Here, we reveal the differences between palmitoyl- and stearoyl-SM in lipid-lipid interactions based on the tie lines obtained from the 2H solid-state NMR spectra of bilayer systems composed of SM/dioleoylphosphatidylcholine/cholesterol 33:33:33 and 40:40:20. Lipid probes carrying position-selective deuterations, 10',10'-d2-SM, 24-d1-cholesterol, and 6″,6″-d2-dioleoyl-phosphatidylcholine, were incorporated into the membranes. 2H NMR peaks from these probes in the membranes directly provide the lipid compositions of the liquid-ordered (Lo) and liquid-disordered (Ld) regions. Without using bulky fluorescent groups, these probes allow us to obtain the end points of the tie lines in a ternary phase diagram based on the lever rule. Consequently, the tie lines of the stearoyl-SM membranes were steeper than those of the palmitoyl-SM membranes, indicating that cholesterol content was higher in the Lo domains of stearoyl-SM, regardless of the total concentration of unsaturated phospholipids. When comparing the content of unsaturated lipids in the Lo domain, the stearoyl-SM membranes had a higher content than palmitoyl-SM membranes. These results revealed that stearoyl-SM is suitable for stabilizing biologically functional microdomains in cholesterol-rich organs, whereas palmitoyl-SM may be better suited for stabilizing domains in tissue membranes with normal cholesterol content. The small but significant differences in the lipid interactions between stearoyl-SM and palmitoyl-SM may be related to the spatiotemporal formation of functional domains in biological environments
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|a Journal Article
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|a Comparative Study
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|a 1,2-oleoylphosphatidylcholine
|2 NLM
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|a EDS2L3ODLV
|2 NLM
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|a Cholesterol
|2 NLM
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|a 97C5T2UQ7J
|2 NLM
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| 650 |
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|a Lipid Bilayers
|2 NLM
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| 650 |
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|a Phosphatidylcholines
|2 NLM
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| 650 |
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|a Sphingomyelins
|2 NLM
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| 700 |
1 |
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|a Yamanaka, Ayana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ibata, Yuki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yasuda, Tomokazu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Umegawa, Yuichi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Murata, Michio
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 40(2024), 43 vom: 29. Okt., Seite 22973-22981
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:40
|g year:2024
|g number:43
|g day:29
|g month:10
|g pages:22973-22981
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|u http://dx.doi.org/10.1021/acs.langmuir.4c03104
|3 Volltext
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|d 40
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