CfSGR1 and CfSGR2 from Cryptomeria fortunei exhibit contrasting responses to hormones and abiotic stress in transgenic Arabidopsis
Copyright © 2024 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 216(2024) vom: 26. Nov., Seite 109152 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article CfSGR1 gene CfSGR2 gene Cryptomeria fortunei Functional study Stress resistance Plant Proteins Plant Growth Regulators Abscisic Acid 72S9A8J5GW |
| Zusammenfassung: | Copyright © 2024 Elsevier Masson SAS. All rights reserved. Stay-green (SGR) genes are pivotal regulatory genes in the context of plant chlorophyll metabolism, but few studies on SGR homologues in Cryptomeria fortunei have been previously reported. We cloned two CfSGR genes and overexpressed them in Arabidopsis to explore their functions. Full-length CfSGR1 and CfSGR2 are 1265 and 1197 bp, encompassing open reading frames (ORFs) encoding 274 and 276 amino acids, respectively. SGRs exhibited high conservation in higher plants, and phylogenetic analysis indicated that SGRs from monocots and gymnosperms cluster in a clade. The proteins localized to chloroplasts and showed no transcriptional activity in yeast cells. The CfSGR gene expressions were induced by abiotic stresses and hormones. Under conditions of darkness, abscisic acid (ABA), salt, drought, or freezing stress, CfSGR2-transgenic Arabidopsis exhibited a delay in leaf yellowing compared to the WT, which was attributed to increased chlorophyll content and enhanced photosynthetic capacity. These transgenic plants exhibited improved resistance to stress via upregulated expression of resistance-related genes, increased antioxidant enzyme activities, and reduced malondialdehyde content and electrolyte leakage rate. In contrast, CfSGR1-transgenic plants may accelerate leaf yellowing and exhibit reduced stress resistance. Our findings highlight potential divergence in the functions of CfSGR genes concerning plant growth and development and responses to abiotic stresses or hormones, providing a scientific foundation for future breeding of stress-resistant C. fortunei cultivars |
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| Beschreibung: | Date Completed 07.11.2024 Date Revised 07.11.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2024.109152 |