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241018s2024 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202407650
|2 doi
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|a pubmed24n1624.xml
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|a (NLM)39420545
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Spiteri, Chantelle
|e verfasserin
|4 aut
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| 245 |
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|a Spatially-Resolved Organoid Transfection by Porous Silicon-Mediated Optoporation
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.12.2024
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|a Date Revised 07.12.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.
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|a Engineering the spatial organisation of organotypic cultures is pivotal for refining tissue models that are useful for gaining deeper insights into complex, non-cell autonomous processes. These advanced models are key to improving the understanding of fundamental biological mechanisms and therapeutic strategies. Controlling gene regulation through spatially-resolved delivery of nucleic acids provides an attractive approach to produce such tissue models. An emerging strategy for spatially-resolved transfection uses photosensitizing nanoparticles coupled with laser pulses to optoporate cells in culture and locally mediate gene delivery. However, localized optoporation in 3D systems remains challenging. Here we propose a solution to this longstanding hurdle, demonstrating that porous silicon nanoparticles are a safe and bioresorbable photosensitising nanomaterial capable of spatially-resolved transfection of mRNA in MCF-7 organoids by near-infrared two-photon optoporation. Functionalization with an azobenzene-lysine photo-switchable moiety enhances the transfection efficiency of the nanoparticles up to 84% in a 2D cell system. Moreover, the nanoparticles enable spatially selective mRNA transfection to MCF-7 spheroids, demonstrating targeted gene delivery in complex 3D cellular environments. The approach for spatially-resolved 3D optoporation offers a way forward for the design of tailored spheroids and organoids by spatially selective nucleic acids delivery
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|a Journal Article
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|a gene delivery
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|a optoporation
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|a organoids
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4 |
|a porous silicon
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| 650 |
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4 |
|a transfection
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| 650 |
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|a Silicon
|2 NLM
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| 650 |
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7 |
|a Z4152N8IUI
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
|2 NLM
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| 700 |
1 |
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|a Caprettini, Valeria
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yikai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dominguez-Gil, Sofia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kaasalainen, Martti
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Cong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martella, Davide Alessandro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a McLennan, Samuel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Vashisth, Priya
|e verfasserin
|4 aut
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1 |
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|a Gary-Bobo, Magali
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nguyen, Christophe
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bergholt, Mads
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Durand, Jean-Olivier
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cunin, Frédérique
|e verfasserin
|4 aut
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| 700 |
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|a Chiappini, Ciro
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 36(2024), 49 vom: 18. Dez., Seite e2407650
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
1 |
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|g volume:36
|g year:2024
|g number:49
|g day:18
|g month:12
|g pages:e2407650
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202407650
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