OsCNGC7 modulates calcium dynamics and accelerates leaf senescence in rice

Copyright © 2024 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 216(2024) vom: 01. Nov., Seite 109193
1. Verfasser: Chang, Jun (VerfasserIn)
Weitere Verfasser: Zhang, Ruxuan, Fu, Zhanmeng, Wang, Yifan, Lei, Jianhui, Cheng, Junyi, Ren, Caihui, Xu, Kunpeng, Gu, Chengzhen, Song, Yuanyuan, Zeng, Rensen, Qin, Yuan, Zhang, Huiying, Liu, Jian
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Calcium Leaf senescence Nuclear envelope OsCNGC7 Rice grain size Plant Proteins SY7Q814VUP Cyclic Nucleotide-Gated Cation Channels
Beschreibung
Zusammenfassung:Copyright © 2024 Elsevier Masson SAS. All rights reserved.
Calcium plays a crucial role in regulating plant senescence. However, the specific effects of increased intranuclear calcium versus cytoplasmic calcium on aging remain unclear. Cyclic nucleotide-gated channels (CNGCs), which manage Ca2⁺ levels in plant cells, are particularly significant in this context. These channels are known to relocate between the nuclear envelope and the plasma membrane in response to stress and developmental signals. Through this movement, CNGCs help regulate the balance of cytosolic and intranuclear Ca2⁺. In this study, we categorized the 16 CNGC genes in rice into five subgroups. OsCNGCs are notably expressed in leaves, especially during the reproductive stage. Both OsCNGC6 and OsCNGC7 exhibit dual localization to the plasma membrane and the nuclear envelope. Knockdown of OsCNGC7 led to reduced levels of Ca2⁺ and K⁺ in plants. Conversely, yeast expressing the OsCNGC7 gene showed increased sensitivity to Ca2⁺. Additionally, while the [Ca2⁺]cyt was maintained at relatively low levels in both wild-type and OsCNGC7-RNAi lines, the fluorescence intensity was significantly higher in OsCNGC7-overexpressing lines, particularly in the nucleus of root tips. Overexpression of OsCNGC7 resulted in enhanced stomatal opening and accelerated leaf senescence from the tillering stage to grain filling in rice. Treatment with MeJA rapidly induced OsCNGC7 expression, while knockdown of OsCNGC7 delayed both MeJA-induced and dark-induced leaf senescence. Further analysis revealed that OsCNGC7 interacts with OsKAT2 and OsALMT2. In conclusion, our findings highlight the distinct roles of OsCNGCs in regulating senescence. This knowledge could provide new strategies for manipulating plant senescence and enhancing crop productivity
Beschreibung:Date Completed 07.11.2024
Date Revised 07.11.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2024.109193