Unlocking sustainable solutions : wood waste as a novel substrate for polyhydroxybutyrate (PHB) production to combat plastic pollution

Polyhydroxybutyrate (PHB) is considered as a hope for bioplastic production, which can serve as a sustainable alternative. Utilizing feedstock as substrate is widely explored for the production but wood waste, which is abundant in cellulose, hemicellulose and lignocellulose, has limited studies for...

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Veröffentlicht in:Environmental technology. - 1993. - (2024) vom: 10. Okt., Seite 1-13
1. Verfasser: Kumar, Mukesh (VerfasserIn)
Weitere Verfasser: Sachan, Rohan Samir Kumar, Kauts, Simran, Karnwal, Arun, Mahmoud, Alaa El Din
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article Plackett-Burman design Polyhydroxyalkanoate Pseudomonas fluorescens polyhydroxybutyrate wood waste
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520 |a Polyhydroxybutyrate (PHB) is considered as a hope for bioplastic production, which can serve as a sustainable alternative. Utilizing feedstock as substrate is widely explored for the production but wood waste, which is abundant in cellulose, hemicellulose and lignocellulose, has limited studies for PHB production. Herein, wood waste is used as a biobased feedstock Hydrolyses of wood waste was done using sulphuric acid (H2SO4) to break down of cellulose and hemicellulose into simple carbon forms. The hydrolysed product was analysed for sugar presence by quantitative and qualitative methods. Pseudomonas fluorescens bacterial strain was used for the production purpose using hydrolysed wood waste as substrate media. The Plackett-Burman design (PBD) and response surface methodology (RSM) were applied to optimize the growth media. The results of PBD were used to identify significant factors influencing PHB production, which were then further optimized using RSM. The work's results conclusively demonstrated that P. fluorescens possesses the capability to effectively utilize wood waste and wastewater as substrate media up to production rate of 13-14 mg mL-1 of PHB. Fourier Transformed Infra-Red (FTIR) spectroscopic peaks confirm the produced product is PHB, which is a type of polyhydroxyalkanoate (PHA), classified within the polyester family highlighting wood waste potential as a sustainable solution to address plastic pollution 
650 4 |a Journal Article 
650 4 |a Plackett-Burman design 
650 4 |a Polyhydroxyalkanoate 
650 4 |a Pseudomonas fluorescens 
650 4 |a polyhydroxybutyrate 
650 4 |a wood waste 
700 1 |a Sachan, Rohan Samir Kumar  |e verfasserin  |4 aut 
700 1 |a Kauts, Simran  |e verfasserin  |4 aut 
700 1 |a Karnwal, Arun  |e verfasserin  |4 aut 
700 1 |a Mahmoud, Alaa El Din  |e verfasserin  |4 aut 
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