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|a 10.1107/S1600576724006101
|2 doi
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|a eng
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|a Wollter, August
|e verfasserin
|4 aut
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|a Coherent X-ray diffraction imaging of single particles
|b background impact on 3D reconstruction
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Revised 11.10.2024
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|a published: Electronic-eCollection
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|a Citation Status PubMed-not-MEDLINE
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|a © Wollter and Ekeberg 2024.
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|a Coherent diffractive imaging with X-ray free-electron lasers could enable structural studies of macromolecules at room temperature. This type of experiment could provide a means to study structural dynamics on the femtosecond timescale. However, the diffraction from a single protein is weak compared with the incoherent scattering from background sources, which negatively affects the reconstruction analysis. This work evaluates the effects of the presence of background on the analysis pipeline. Background measurements from the European X-ray Free-Electron Laser were combined with simulated diffraction patterns and treated by a standard reconstruction procedure, including orientation recovery with the expand, maximize and compress algorithm and 3D phase retrieval. Background scattering did have an adverse effect on the estimated resolution of the reconstructed density maps. Still, the reconstructions generally worked when the signal-to-background ratio was 0.6 or better, in the momentum transfer shell of the highest reconstructed resolution. The results also suggest that the signal-to-background requirement increases at higher resolution. This study gives an indication of what is possible at current setups at X-ray free-electron lasers with regards to expected background strength and establishes a target for experimental optimization of the background
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|a Journal Article
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|a EMC
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|a X-ray free-electron lasers
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|a background noise
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| 650 |
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|a coherent diffractive imaging
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| 650 |
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|a phase retrieval
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| 650 |
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4 |
|a structural biology
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| 700 |
1 |
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|a Ekeberg, Tomas
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of applied crystallography
|d 1998
|g 57(2024), Pt 5 vom: 01. Okt., Seite 1384-1391
|w (DE-627)NLM098121561
|x 0021-8898
|7 nnns
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| 773 |
1 |
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|g volume:57
|g year:2024
|g number:Pt 5
|g day:01
|g month:10
|g pages:1384-1391
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|u http://dx.doi.org/10.1107/S1600576724006101
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|d 57
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|h 1384-1391
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