|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM378736116 |
| 003 |
DE-627 |
| 005 |
20241011232831.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
241010s2024 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1107/S1600576724008987
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1564.xml
|
| 035 |
|
|
|a (DE-627)NLM378736116
|
| 035 |
|
|
|a (NLM)39387087
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Nepal, Prakash
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Characterization of sub-micrometre-sized voids in fixed human brain tissue using scanning X-ray microdiffraction
|
| 264 |
|
1 |
|c 2024
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 11.10.2024
|
| 500 |
|
|
|a published: Electronic-eCollection
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a © Prakash Nepal et al. 2024.
|
| 520 |
|
|
|a Using a 5 µm-diameter X-ray beam, we collected scanning X-ray microdiffraction in both the small-angle (SAXS) and the wide-angle (WAXS) regimes from thin sections of fixed human brain tissue from Alzheimer's subjects. The intensity of scattering in the SAXS regime of these patterns exhibits essentially no correlation with the observed intensity in the WAXS regime, indicating that the structures responsible for these two portions of the diffraction patterns, which reflect different length scales, are distinct. SAXS scattering exhibits a power-law behavior in which the log of intensity decreases linearly with the log of the scattering angle. The slope of the log-log curve is roughly proportional to the intensity in the SAXS regime and, surprisingly, inversely proportional to the intensity in the WAXS regime. We interpret these observations as being due to the presence of sub-micrometre-sized voids formed during dehydration of the fixed tissue. The SAXS intensity is due largely to scattering from these voids, while the WAXS intensity derives from the secondary structures of macromolecular material surrounding the voids. The ability to detect and map the presence of voids within thin sections of fixed tissue has the potential to provide novel information on the degradation of human brain tissue in neurodegenerative diseases
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a SAXS
|
| 650 |
|
4 |
|a WAXS
|
| 650 |
|
4 |
|a X-ray microdiffraction
|
| 650 |
|
4 |
|a in situ structural studies
|
| 650 |
|
4 |
|a neurodegenerative diseases
|
| 650 |
|
4 |
|a small-angle X-ray scattering
|
| 650 |
|
4 |
|a wide-angle X-ray scattering
|
| 700 |
1 |
|
|a Bashit, Abdullah A
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Makowski, Lee
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of applied crystallography
|d 1998
|g 57(2024), Pt 5 vom: 01. Okt., Seite 1528-1538
|w (DE-627)NLM098121561
|x 0021-8898
|7 nnns
|
| 773 |
1 |
8 |
|g volume:57
|g year:2024
|g number:Pt 5
|g day:01
|g month:10
|g pages:1528-1538
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1107/S1600576724008987
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 57
|j 2024
|e Pt 5
|b 01
|c 10
|h 1528-1538
|