Synergistic effect of Hypoxic Conditioning and Cell-Tethering Colloidal Gels enhanced Productivity of MSC Paracrine Factors and Accelerated Vessel Regeneration

Bibliographische Detailangaben
Veröffentlicht in:	Advanced materials (Deerfield Beach, Fla.). - 1998. - (2024) vom: 09. Okt., Seite e2408488
1. Verfasser:	Lee, Myung Chul (VerfasserIn)
Weitere Verfasser:	Lee, Jae Seo, Kim, Seongsoo, Jamaiyar, Anurag, Wu, Winona, Gonzalez, Montserrat Legorreta, Durán, Tania Carolina Acevedo, Madrigal-Salazar, Andrea Donaxi, Bassous, Nicole, Carvalho, Violeta, Choi, Cholong, Kim, Da-Seul, Seo, Jeong Wook, Rodrigues, Nelson, Teixeira, Senhorinha F C F, Alkhateeb, Abdulhameed F, Soto, Javier Alejandro Lozano, Hussain, Mohammad Asif, Leijten, Jeroen, Feinberg, Mark W, Shin, Su Ryon
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Advanced materials (Deerfield Beach, Fla.)
Schlagworte:	Journal Article colloidal gel hMSC hypoxic conditioning ischemic disease mechanical stimulation oxygenating microparticles paracrine effect vessel regeneration

Beschreibung
Zusammenfassung:	© 2024 Wiley‐VCH GmbH. Microporous hydrogels have been widely used for delivering therapeutic cells. However, several critical issues, such as the lack of control over the harsh environment they are subjected to under pathological conditions and rapid egression of cells from the hydrogels, have produced limited therapeutic outcomes. To address these critical challenges, cell-tethering and hypoxic conditioning colloidal hydrogels containing mesenchymal stem cells (MSCs) are introduced to increase the productivity of paracrine factors locally and in a long-term manner. Cell-tethering colloidal hydrogels that are composed of tyramine-conjugated gelatin prevent cells from egressing through on-cell oxidative phenolic crosslinks while providing mechanical stimulation and interconnected microporous networks to allow for host-implant interactions. Oxygenating microparticles encapsulated in tyramine-conjugated colloidal microgels continuously generated oxygen for 2 weeks with rapid diffusion, resulting in maintaining a mild hypoxic condition while MSCs consumed oxygen under severe hypoxia. Synergistically, local retention of MSCs within the mild hypoxic-conditioned and mechanically robust colloidal hydrogels significantly increased the secretion of various angiogenic cytokines and chemokines. The oxygenating colloidal hydrogels induced anti-inflammatory responses, reduced cellular apoptosis, and promoted numerous large blood vessels in vivo. Finally, mice injected with the MSC-tethered oxygenating colloidal hydrogels significantly improved blood flow restoration and muscle regeneration in a hindlimb ischemia (HLI) model
Beschreibung:	Date Revised 09.10.2024 published: Print-Electronic Citation Status Publisher
ISSN:	1521-4095
DOI:	10.1002/adma.202408488