Genomic and biochemical analyses of lipid biosynthesis in Cyanophora paradoxa : limited role of the chloroplast in fatty acid synthesis
© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.
| Publié dans: | Journal of experimental botany. - 1985. - 76(2025), 2 vom: 10. Jan., Seite 532-545 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Journal of experimental botany |
| Sujets: | Journal Article Cyanophora paradoxa Acetyl-CoA carboxylase carbon isotope assimilation chloroplast comparative genomics fatty acid synthesis Fatty Acids |
| Résumé: | © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. Archaeplastida, a group of photosynthetic organisms with primary plastids, consists of green algae (plus land plants), red algae, and glaucophytes. In contrast to green and red algae, information on lipids and lipid biosynthesis is still incomplete in the glaucophytes. The chloroplast is the site of photosynthesis and fatty acid synthesis in all photosynthetic organisms known to date. However, the genomic data of the glaucophyte Cyanophora paradoxa indicated the lack of acetyl-CoA carboxylase and most components of fatty acid synthase in the chloroplast. Instead, multifunctional fatty acid synthase and acetyl-CoA carboxylase are likely to reside in the cytosol. To examine this hypothesis, we measured fatty acid synthesis in isolated chloroplasts and whole cells using stable isotope labeling. The chloroplasts had very low fatty acid synthesis activity, if any. Most processes of fatty acid synthesis, including elongation and desaturation, must be performed within the cytosol, and the fatty acids imported into the chloroplasts are assembled into the chloroplast lipids by the enzymes common to other algae and plants. Cyanophora paradoxa is a rare organism in which fatty acid synthesis and photosynthesis are not tightly linked. This could question the common origin of these two biosynthetic processes in Archaeplastida |
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| Description: | Date Completed 09.01.2025 Date Revised 11.01.2025 published: Print Citation Status MEDLINE |
| ISSN: | 1460-2431 |
| DOI: | 10.1093/jxb/erae420 |