|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM378438352 |
| 003 |
DE-627 |
| 005 |
20250306175206.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
241003s2024 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2024.109137
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1260.xml
|
| 035 |
|
|
|a (DE-627)NLM378438352
|
| 035 |
|
|
|a (NLM)39357201
|
| 035 |
|
|
|a (PII)S0981-9428(24)00805-2
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Takáč, Tomáš
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Actin cytoskeleton and plasma membrane aquaporins are involved in different drought response of Arabidopsis rhd2 and der1 root hair mutants
|
| 264 |
|
1 |
|c 2024
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 07.11.2024
|
| 500 |
|
|
|a Date Revised 07.11.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
|
| 520 |
|
|
|a Actin cytoskeleton and reactive oxygen species are principal determinants of root hair polarity and tip growth. Loss of function in RESPIRATORY BURST OXIDASE HOMOLOG C/ROOT HAIR DEFECTIVE 2 (AtRBOHC/RHD2), an NADPH oxidase emitting superoxide to the apoplast, and in ACTIN 2, a vegetative actin isovariant, in rhd2-1 and der1-3 mutants, respectively, lead to similar defects in root hair formation and elongation Since early endosome-mediated polar localization of AtRBOHC/RHD2 depends on actin cytoskeleton, comparing the proteome-wide consequences of both mutations might be of eminent interest. Therefore, we employed a differential proteomic analysis of Arabidopsis rhd2-1 and der1-3 mutants. Both mutants exhibited substantial alterations in abundances of stress-related proteins. Notably, plasma membrane (PM)-localized PIP aquaporins showed contrasting abundance patterns in the mutants compared to wild-types. Drought-responsive proteins were mostly downregulated in rhd2-1 but upregulated in der1-3. Proteomic data suggest that opposite to der1-3, altered vesicular transport in rhd2-1 mutant likely contributes to the deregulation of PM-localized proteins, including PIPs. Moreover, lattice light sheet microscopy revealed reduced actin dynamics in rhd2-1 roots, a finding contrasting with previous reports on der1-3 mutant. Phenotypic experiments demonstrated a drought stress susceptibility in rhd2-1 and resistance in der1-3. Thus, mutations in AtRBOHC/RHD2 and ACTIN2 cause similar root hair defects, but they differently affect the actin cytoskeleton and vesicular transport. Reduced actin dynamics in rhd2-1 mutant is accompanied by alteration of vesicular transport proteins abundance, likely leading to altered protein delivery to PM, including aquaporins, thereby significantly affecting drought stress responses
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a ACTIN2
|
| 650 |
|
4 |
|a Aquaporin
|
| 650 |
|
4 |
|a Arabidopsis
|
| 650 |
|
4 |
|a Drought stress
|
| 650 |
|
4 |
|a NADPH oxidase
|
| 650 |
|
4 |
|a PIP1
|
| 650 |
|
4 |
|a Plasma membrane
|
| 650 |
|
4 |
|a Proteomics
|
| 650 |
|
4 |
|a RBOHC
|
| 650 |
|
4 |
|a actin
|
| 650 |
|
7 |
|a Arabidopsis Proteins
|2 NLM
|
| 650 |
|
7 |
|a Aquaporins
|2 NLM
|
| 700 |
1 |
|
|a Kuběnová, Lenka
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Šamajová, Olga
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dvořák, Petr
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Řehák, Jan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Haberland, Jan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bundschuh, Sebastian T
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Pechan, Tibor
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Tomančák, Pavel
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ovečka, Miroslav
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Šamaj, Jozef
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 216(2024) vom: 08. Nov., Seite 109137
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
|
| 773 |
1 |
8 |
|g volume:216
|g year:2024
|g day:08
|g month:11
|g pages:109137
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2024.109137
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 216
|j 2024
|b 08
|c 11
|h 109137
|