DNAMarkMaker : streamlining ARMS and CAPS marker development from resequencing data with NGS short reads
Copyright © 2024 by JAPANESE SOCIETY OF BREEDING.
Veröffentlicht in: | Breeding science. - 1998. - 74(2024), 2 vom: 01. Apr., Seite 73-82 |
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1. Verfasser: | |
Weitere Verfasser: | , , , , , |
Format: | Online-Aufsatz |
Sprache: | English |
Veröffentlicht: |
2024
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Zugriff auf das übergeordnete Werk: | Breeding science |
Schlagworte: | Journal Article ARMS CAPS DNA marker Next-generation sequencing SNP |
Zusammenfassung: | Copyright © 2024 by JAPANESE SOCIETY OF BREEDING. DNA markers serve as essential tools in breeding selection and genetic analysis. However, developing DNA markers can be time-consuming and labor-intensive due to the need to identify polymorphisms between cultivars/lines and to design suitable primers. To address these challenges, we have developed DNAMarkMaker, a tool designed to automate the process of primer design for Amplification Refractory Mutation System (ARMS) and Cleaved Amplified Polymorphic Sequences (CAPS) markers, utilizing resequencing data. One key feature of DNAMarkMaker is its user-friendly graphical user interface (GUI), ensuring its accessibility and ease of use, even for researchers not well-versed in bioinformatics. We confirmed DNAMarkMaker's applicability by developing DNA markers for rice, potato, and turnip-each representing distinct genome structures: homozygous diploid, heterozygous autotetraploid, and heterozygous diploid, respectively. DNAMarkMaker will contribute to the rapid and efficient development of DNA markers, accelerating breeding and genetic analysis in various crops |
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Beschreibung: | Date Revised 02.10.2024 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
ISSN: | 1344-7610 |
DOI: | 10.1270/jsbbs.23048 |