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240923s2024 xx |||||o 00| ||eng c |
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|a 10.1111/nph.20141
|2 doi
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|a pubmed24n1546.xml
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wang, Lei
|e verfasserin
|4 aut
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| 245 |
1 |
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|a CRISPR/Cas9-based editing of NF-YC4 promoters yields high-protein rice and soybean
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Revised 22.09.2024
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
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|a Genome editing is a revolution in biotechnology for crop improvement with the final product lacking transgenes. However, most derived traits have been generated through edits that create gene knockouts. Our study pioneers a novel approach, utilizing gene editing to enhance gene expression by eliminating transcriptional repressor binding motifs. Building upon our prior research demonstrating the protein-boosting effects of the transcription factor NF-YC4, we identified conserved motifs targeted by RAV and WRKY repressors in the NF-YC4 promoters from rice (Oryza sativa) and soybean (Glycine max). Leveraging CRISPR/Cas9 technology, we deleted these motifs, resulting in reduced repressor binding and increased NF-YC4 expression. This strategy led to increased protein content and reduced carbohydrate levels in the edited rice and soybean plants, with rice exhibiting up to a 68% increase in leaf protein and a 17% increase in seed protein, and soybean showing up to a 25% increase in leaf protein and an 11% increase in seed protein. Our findings provide a blueprint for enhancing gene expression through precise genomic deletions in noncoding sequences, promising improved agricultural productivity and nutritional quality
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|a Journal Article
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|a CRISPR/Cas9
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|a NF‐YC4 promoter
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|a protein
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| 650 |
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|a rice
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| 650 |
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4 |
|a soybean
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1 |
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|a O'Conner, Seth
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tanvir, Rezwan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zheng, Wenguang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cothron, Samuel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Towery, Katherine
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bi, Honghao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ellison, Evan E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Bing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Voytas, Daniel F
|e verfasserin
|4 aut
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| 700 |
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|a Li, Ling
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g (2024) vom: 22. Sept.
|w (DE-627)NLM09818248X
|x 1469-8137
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|g year:2024
|g day:22
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|u http://dx.doi.org/10.1111/nph.20141
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