A nitrogen-responsive cytokinin oxidase/dehydrogenase regulates root response to high ammonium in rice

A nitrogen-responsive cytokinin oxidase/dehydrogenase regulates root response to high ammonium in rice

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 244(2024), 4 vom: 17. Okt., Seite 1391-1407
1. Verfasser: Li, Lun (VerfasserIn)
Weitere Verfasser: Jia, Letian, Duan, Xingliang, Lv, Yuanda, Ye, Chengyu, Ding, Chengqiang, Zhang, Yuwen, Qi, Weicong, Motte, Hans, Beeckman, Tom, Luo, Le, Xuan, Wei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article CKX ammonium auxin cytokinin lateral root rice seminal root cytokinin oxidase EC 1.5.99.12 mehr... Ammonium Compounds Cytokinins Nitrogen N762921K75 Oxidoreductases EC 1.- Plant Proteins Indoleacetic Acids
Beschreibung
Zusammenfassung:© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
Plant root system is significantly influenced by high soil levels of ammonium nitrogen, leading to reduced root elongation and enhanced lateral root branching. In Arabidopsis, these processes have been reported to be mediated by phytohormones and their downstream signaling pathways, while the controlling mechanisms remain elusive in crops. Through a transcriptome analysis of roots subjected to high/low ammonium treatments, we identified a cytokinin oxidase/dehydrogenase encoding gene, CKX3, whose expression is induced by high ammonium. Knocking out CKX3 and its homologue CKX8 results in shorter seminal roots, fewer lateral roots, and reduced sensitivity to high ammonium. Endogenous cytokinin levels are elevated by high ammonium or in ckx3 mutants. Cytokinin application results in shorter seminal roots and fewer lateral roots in wild-type, mimicking the root responses of ckx3 mutants to high ammonium. Furthermore, CKX3 is transcriptionally activated by type-B RR25 and RR26, and ckx3 mutants have reduced auxin content and signaling in roots under low ammonium. This study identified RR25/26-CKX3-cytokinin as a signal module that mediates root responses to external ammonium by modulating of auxin signaling in the root meristem and lateral root primordium. This highlights the critical role of cytokinin metabolism in regulating rice root development in response to ammonium
Beschreibung:Date Completed 17.10.2024
Date Revised 17.10.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.20128