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240918s2024 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.4c02198
|2 doi
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|a pubmed24n1554.xml
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|a (DE-627)NLM377750417
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|a (NLM)39288013
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Xie, Wenting
|e verfasserin
|4 aut
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|a Extraction of Genomic DNA from Soil Samples by Polyethylene Glycol-Modified Magnetic Particles via Isopropanol Promotion and Ca2+ Mediation
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.10.2024
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|a Date Revised 01.10.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Obtaining reliable and informative DNA data from soil samples is challenging due to the presence of interfering substances and typically low DNA yields. In this work, we prepared poly(ethylene glycol)-modified magnetic particles (PEGFe3O4) for DNA purification. The particles leverage the facilitative effect of calcium ions (Ca2+), which act as bridges between DNA and PEG@Fe3O4 by coordinating with the phosphate groups of DNA and the hydroxyl groups on the particles. The addition of 2-propanol further enhances this Ca2+-mediated DNA adsorption by inducing a conformational change from the B-form to the more compact A-form of DNA. PEG@Fe3O4 demonstrates a DNA adsorption capacity of 144.6 mg g-1. When applied to the extraction of genomic DNA from soil samples, PEG@Fe3O4 outperforms commercial kits and traditional phenol-chloroform extraction methods in terms of DNA yield and purity. Furthermore, we developed a 16-channel automated DNA extraction device to streamline the process and reduce the extraction time. The successful amplification of target bacterial and fungal amplicons underscores the potential of this automated, device-assisted method for studying soil microbial diversity
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|a Journal Article
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|a Polyethylene Glycols
|2 NLM
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|a 3WJQ0SDW1A
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|a 2-Propanol
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|a ND2M416302
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|a Calcium
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|a SY7Q814VUP
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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|a Soil
|2 NLM
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|a DNA, Bacterial
|2 NLM
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|a Xue, Jialiang
|e verfasserin
|4 aut
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|a Chen, Ruobo
|e verfasserin
|4 aut
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| 700 |
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|a Su, Huihui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Xun
|e verfasserin
|4 aut
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| 700 |
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|a Wu, Qingxi
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Wenjuan
|e verfasserin
|4 aut
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|a Jia, Li
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 40(2024), 39 vom: 01. Okt., Seite 20550-20558
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:40
|g year:2024
|g number:39
|g day:01
|g month:10
|g pages:20550-20558
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|u http://dx.doi.org/10.1021/acs.langmuir.4c02198
|3 Volltext
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