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240917s2024 xx |||||o 00| ||eng c |
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|a 10.1111/nph.20133
|2 doi
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|a pubmed24n1581.xml
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|a (DE-627)NLM377725579
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|a (NLM)39285533
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Kamileen, Mohamed O
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Streamlined screening platforms lead to the discovery of pachysiphine synthase from Tabernanthe iboga
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| 264 |
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1 |
|c 2024
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.10.2024
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|a Date Revised 25.10.2024
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|a published: Print-Electronic
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|a RefSeq: AF063850.1, PQ178877, PQ178876
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|a Citation Status MEDLINE
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|a © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
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|a Plant-specialized metabolism is largely driven by the oxidative tailoring of key chemical scaffolds catalyzed by cytochrome P450 (CYP450s) enzymes. Monoterpene indole alkaloids (MIAs) tabersonine and pseudo-tabersonine, found in the medicinal plant Tabernanthe iboga (commonly known as iboga), are tailored with oxidations, and the enzymes involved remain unknown. Here, we developed a streamlined screening strategy to test the activity of T. iboga CYP450s in Nicotiana benthamiana. Using multigene constructs encoding the biosynthesis of tabersonine and pseudo-tabersonine scaffolds, we aimed to uncover the CYP450s responsible for oxidative transformations in these scaffolds. Our approach identified two T. iboga cytochrome P450 enzymes: pachysiphine synthase (PS) and 16-hydroxy-tabersonine synthase (T16H). These enzymes catalyze an epoxidation and site-specific hydroxylation of tabersonine to produce pachysiphine and 16-OH-tabersonine, respectively. This work provides new insights into the biosynthetic pathways of MIAs and underscores the utility of N. benthamiana and Catharanthus roseus as platforms for the functional characterization of plant enzymes
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4 |
|a Journal Article
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|a Nicotiana benthamiana
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| 650 |
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4 |
|a Tabernanthe iboga
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| 650 |
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4 |
|a alkaloid
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| 650 |
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4 |
|a cytochrome P450
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| 650 |
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4 |
|a pachysiphine
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| 650 |
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4 |
|a plant biosynthesis
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| 650 |
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4 |
|a plant natural product
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| 650 |
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7 |
|a Cytochrome P-450 Enzyme System
|2 NLM
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| 650 |
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7 |
|a 9035-51-2
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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7 |
|a Indole Alkaloids
|2 NLM
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| 650 |
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7 |
|a tabersonine
|2 NLM
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| 650 |
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7 |
|a 4429-63-4
|2 NLM
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| 650 |
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7 |
|a Quinolines
|2 NLM
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| 700 |
1 |
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|a Nakamura, Yoko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luck, Katrin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Heinicke, Sarah
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hong, Benke
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Colinas, Maite
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lichman, Benjamin R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a O'Connor, Sarah E
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 244(2024), 4 vom: 16. Okt., Seite 1437-1449
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
8 |
|g volume:244
|g year:2024
|g number:4
|g day:16
|g month:10
|g pages:1437-1449
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| 856 |
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|u http://dx.doi.org/10.1111/nph.20133
|3 Volltext
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|a GBV_ILN_350
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|a AR
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|d 244
|j 2024
|e 4
|b 16
|c 10
|h 1437-1449
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