A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein

Avidin-biotin binding, the most robust non-covalent protein-ligand interaction occurring in nature, has wide-ranging applications in biotechnology. A frequent challenge in these applications is accurately determining the number of unoccupied biotin binding sites in avidin-containing fusion proteins....

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Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques. - 1991. - 76(2024), 10 vom: 07. Okt., Seite 485-494
1. Verfasser:	Mukherjee, Sonia (VerfasserIn)
Weitere Verfasser:	Leblanc, Pierre, Poznansky, Mark C, Sluder, Ann E
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article avidin binding assay binding site occupancy biotin fusion protein self-assembling vaccine Biotin 6SO6U10H04 Avidin mehr... 1405-69-2 Recombinant Fusion Proteins Coloring Agents Azo Compounds