Genome-wide analysis of the trihelix gene family reveals that MaGT21 modulates fruit ripening by regulating the expression of MaACO1 in Musa acuminata

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 216(2024) vom: 11. Sept., Seite 109089
1. Verfasser:	Liu, Fan (VerfasserIn)
Weitere Verfasser:	Sun, Xueli, Sheng, Ou, Dou, Tongxin, Yang, Qiaosong, Hu, Chunhua, Gao, Huijun, He, Weidi, Deng, Guiming, Dong, Tao, Li, Chunyu, Liu, Siwen, Yi, Ganjun, Bi, Fangcheng
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Banana Fruit ripening MaACO1 MaGT21 Trihelix transcription factors

Beschreibung
Zusammenfassung:	Copyright © 2024 Elsevier Masson SAS. All rights reserved. The trihelix transcription factor (GT) gene family members play vital roles in plant growth and development, responses to abiotic or biotic stress, and fruit ripening. However, its role in banana fruit ripening remains unclear. Here, 59 MaGT gene members were identified in banana and clustered into five subfamilies, namely GT1, GT2, GTγ, SIP1, and SH4. This classification is completely supported by their gene structures and conserved motif analysis. Transcriptome data analysis indicated that MaGT14, MaGT21, and MaGT27 demonstrated significant differential expression during fruit ripening. Quantitative real-time PCR analysis revealed that these three genes were highly induced by ethylene treatment, responded to cold and heat stress, and had a high expression abundance in ripe fruit. Subcellular localization demonstrated that MaGT21 and MaGT27 functioned as nuclear proteins, while MaGT14 functioned as a nuclear and cell membrane protein. Further investigation indicated MaGT21 could positively stimulate the transcription of the key ethylene biosynthesis gene MaACO1 by directly targeting the GT motif in the promoter. MaGT21 transient overexpression in banana fruit upregulated MaACO1 and accelerated fruit ripening. Our findings provide comprehensive and valuable information for further functional studies of MaGT genes in banana, help to understand the roles of MaGTs during banana fruit ripening
Beschreibung:	Date Revised 12.09.2024 published: Print-Electronic Citation Status Publisher
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2024.109089