Rapid achievement of partial nitrification process by adopting the combined strategy of anoxic starvation and free ammonia inhibition

Partial nitrification (PN) is a prerequisite step for the short-cut nitrogen removal process, which is crucial to provide stable nitrite accumulation for subsequent units. The present study innovatively proposed a new strategy for the rapid establishment of PN by adopting short-term anoxic starvatio...

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Veröffentlicht in:Environmental technology. - 1993. - 46(2025), 9 vom: 01. Apr., Seite 1519-1530
1. Verfasser: Wang, Wenxiao (VerfasserIn)
Weitere Verfasser: Zhang, Xiaojing, Ma, Bingbing, Zhang, Han, Wang, Qiong, Song, Yali, Ma, Yongpeng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article Nitrite accumulation ammonia-oxidizing bacteria anoxic starvation free ammonia shortcut nitrogen removal Ammonia 7664-41-7 Sewage Wastewater mehr... ammonia monooxygenase EC 1.7.3.- Nitrites Nitrogen N762921K75 Oxidoreductases EC 1.-
Beschreibung
Zusammenfassung:Partial nitrification (PN) is a prerequisite step for the short-cut nitrogen removal process, which is crucial to provide stable nitrite accumulation for subsequent units. The present study innovatively proposed a new strategy for the rapid establishment of PN by adopting short-term anoxic starvation combined with high free ammonia inhibition. The sludge obtained from the secondary sedimentation tank of a municipal wastewater treatment plant was starved for 7 days under anoxic conditions, and then wastewater with high ammonia nitrogen (400 mg L-1) was introduced. Within 17 days, stable nitrite accumulation was achieved in the sequencing batch reactor, and the nitrite accumulation rate reached more than 95.0%. The activity of ammonia monooxygenase enzyme increased from 0.0364 ± 0.0074 to 0.1275 ± 0.0021 μg NO2--N·mg-1 protein min-1, while that of hydroxylamine oxidoreductase enzyme increased from 1.5350 ± 0.0208 to 6.3852 ± 0.0400 EU g-1 SS. The relative abundance of Nitrosomonas increased from 0.10% to 25.90%, while that of Nitrospira consistently remained below 0.04%. And the relative abundance of short-cut denitrifying bacteria, including Truepera, OLB8, and OLB13 all increased. The results proved that the short-term anoxic starvation combined with high free ammonia inhibition was an effective strategy for rapid establishment of PN
Beschreibung:Date Completed 23.04.2025
Date Revised 23.04.2025
published: Print-Electronic
Citation Status MEDLINE
ISSN:1479-487X
DOI:10.1080/09593330.2024.2401645