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20241015232308.0 |
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240910s2024 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.14011
|2 doi
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|a pubmed24n1568.xml
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|a (DE-627)NLM377395145
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|a (NLM)39252455
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Balan, Rubicely
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Insights on the virulence and genomic features of Lactococcus garvieae isolated from giant freshwater prawn Macrobrachium rosenbergii (de Man 1879)
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.10.2024
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|a Date Revised 14.10.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 John Wiley & Sons Ltd.
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|a Giant freshwater prawn (Macrobrachium rosenbergii (MR)) is a significant aquafarm species commercially cultured in Taiwan. Intensive farming practices have led to the outbreak of Lactococcus garvieae (LG), which causes Lactococcosis in MR. Recently, LG has re-emerged and the number of mortalities in prawn farms has increased in Taiwan. However, there is no preventative strategy described and a lack of knowledge on virulence factors and pathogenesis from LG in MR. The most virulent strain of L. garvieae from M. rosenbergii was screened in vivo among seven isolates selected for infectivity testing injecting 0.1 mL of 108 CFU/mL bacterial concentration. Among the seven isolates screened, L. garvieae 109-6 resulted in 100% mortality within 3 days post-infection. Furthermore, 109-6 L. garvieae LD50 dosage from in MR was found to be 106 CFU/mL. Subsequently, the most virulent strain 109-6 was sequenced using MinIon Nanopore sequencing. Results indicated that the LG genome yielded a protein-coding of 3857 with 59 tRNA and 16 rRNA and no plasmid. Interestingly, the distribution of subsystems in the annotated genome revealed genes related to virulence, defence, and disease among LG 50 genes. Altogether, the virulent strain and its genome data revealed distinctive features of LG, which hinted toward its pathogenicity and could facilitate for better preventive strategies
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|a Journal Article
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|a Lactococcus garvieae
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| 650 |
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|a Macrobrachium rosenbergii
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| 650 |
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|a MinIon Nanopore
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| 650 |
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4 |
|a genome
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| 650 |
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4 |
|a pathogenicity
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| 650 |
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|a Virulence Factors
|2 NLM
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| 700 |
1 |
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|a Pandey, Sudarshan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Pei-Chi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Byadgi, Omkar Vijay
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Shih-Chu
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 47(2024), 11 vom: 09. Okt., Seite e14011
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
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|g volume:47
|g year:2024
|g number:11
|g day:09
|g month:10
|g pages:e14011
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| 856 |
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|u http://dx.doi.org/10.1111/jfd.14011
|3 Volltext
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|a AR
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|d 47
|j 2024
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