Cas12a and MAD7, genome editing tools for breeding
Copyright © 2024 by JAPANESE SOCIETY OF BREEDING.
| Veröffentlicht in: | Breeding science. - 1998. - 74(2024), 1 vom: 20. März, Seite 22-31 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | Breeding science |
| Schlagworte: | Journal Article CRISPR Cas12a MAD7 ST8 breeding genome editing |
| Zusammenfassung: | Copyright © 2024 by JAPANESE SOCIETY OF BREEDING. Food shortages due to population growth and climate change are expected to occur in the near future as a problem that urgently requires solutions. Conventional breeding techniques, notably crossbreeding and mutation breeding, are known for being inefficient and time-consuming in obtaining seeds and seedlings with desired traits. Thus, there is an urgent need for novel methods for efficient plant breeding. Breeding by genome editing is receiving substantial attention because it can efficiently modify the target gene to obtain desired traits compared with conventional methods. Among the programmable sequence-specific nucleases that have been developed for genome editing, CRISPR-Cas12a and CRISPR-MAD7 nucleases are becoming more broadly adopted for the application of genome editing in grains, vegetables and fruits. Additionally, ST8, an improved variant of MAD7, has been developed to enhance genome editing efficiency and has potential for application to breeding of crops |
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| Beschreibung: | Date Revised 10.09.2024 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1344-7610 |
| DOI: | 10.1270/jsbbs.23049 |