Serum chemistry, thyroxine concentration, and blood cell morphology of wild, young Blackchin Guitarfish Glaucostegus cemiculus sampled on the eastern Mediterranean coastline

© 2024 American Fisheries Society.

Bibliographische Detailangaben
Veröffentlicht in:Journal of aquatic animal health. - 1998. - 36(2024), 3 vom: 25. Sept., Seite 281-299
1. Verfasser: Schwartz, Nevo (VerfasserIn)
Weitere Verfasser: Morick, Danny, Azrieli, Barak, Scheinin, Aviad, Tchernov, Dan, Aroch, Itamar
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Journal of aquatic animal health
Schlagworte:Journal Article Elasmobranchii Rajiformes albumin globulin serum protein electrophoresis shark thyroid hormone Thyroxine Q51BO43MG4 Serum Albumin
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245 1 0 |a Serum chemistry, thyroxine concentration, and blood cell morphology of wild, young Blackchin Guitarfish Glaucostegus cemiculus sampled on the eastern Mediterranean coastline 
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520 |a OBJECTIVE: The Blackchin Guitarfish Glaucostegus cemiculus is endemic to the Mediterranean Sea and is critically endangered, but relevant routine laboratory data are unavailable. Our objectives were to determine the packed cell volume (PCV), comprehensive serum chemistry analytes, and serum total thyroxine (sTT4) concentration; compare serum albumin and serum globulin concentrations as measured by two different methods; and describe the blood cell morphology of healthy, free-ranging Blackchin Guitarfish 
520 |a METHODS: Wild Blackchin Guitarfish were captured using a seine net. Blood samples for serum chemistry and hematological analyses were obtained and measured using routine laboratory methods. The fish were tagged and released 
520 |a RESULT: This study included 43 Blackchin Guitarfish (17 males and 26 females) that were younger than 6 months as estimated based on total length and body weight. The median PCV (n = 23) was 22% (minimum-maximum [min-max] = 15-25%). Median sTT4 (n = 10) measured by chemiluminescence immunoassay was 7.86 nmol/L (min-max = 7.52-9.57 nmol/L). The study included a comprehensive, 25-analyte serum chemistry analysis (e.g., serum iron and unbound and total iron-binding capacity) and a morphological description of all blood cells. Serum electrophoresis (SEP; n = 13) yielded a consistent serum albumin-migrating protein fraction and four globulin fractions. Serum electrophoretograms corroborating these results are presented 
520 |a CONCLUSION: In Blackchin Guitarfish, the serum albumin-migrating fraction measured by SEP combined with serum total protein concentration yields a much higher albumin concentration compared to that measured by bromocresol green spectrophotometry. The true identity of this albumin-migrating fraction remains to be identified. The analytes' calculated 2.5-97.5% interpercentile intervals should be considered as reference intervals applying to Blackchin Guitarfish of similar age but should be applied cautiously to adult fish 
650 4 |a Journal Article 
650 4 |a Elasmobranchii 
650 4 |a Rajiformes 
650 4 |a albumin 
650 4 |a globulin 
650 4 |a serum protein electrophoresis 
650 4 |a shark 
650 4 |a thyroid hormone 
650 7 |a Thyroxine  |2 NLM 
650 7 |a Q51BO43MG4  |2 NLM 
650 7 |a Serum Albumin  |2 NLM 
700 1 |a Morick, Danny  |e verfasserin  |4 aut 
700 1 |a Azrieli, Barak  |e verfasserin  |4 aut 
700 1 |a Scheinin, Aviad  |e verfasserin  |4 aut 
700 1 |a Tchernov, Dan  |e verfasserin  |4 aut 
700 1 |a Aroch, Itamar  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of aquatic animal health  |d 1998  |g 36(2024), 3 vom: 25. Sept., Seite 281-299  |w (DE-627)NLM098202006  |x 1548-8667  |7 nnas 
773 1 8 |g volume:36  |g year:2024  |g number:3  |g day:25  |g month:09  |g pages:281-299 
856 4 0 |u http://dx.doi.org/10.1002/aah.10228  |3 Volltext 
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