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NLM377054267 |
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20241104232450.0 |
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240902s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2024.112228
|2 doi
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|a pubmed24n1590.xml
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|a (DE-627)NLM377054267
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|a (NLM)39218307
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|a (PII)S0168-9452(24)00255-3
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Gao, Zihan
|e verfasserin
|4 aut
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| 245 |
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|a The knockout of SlMTC impacts tomato seed size and reduces resistance to salt stress in tomato
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|c 2024
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 27.10.2024
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|a Date Revised 04.11.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier B.V. All rights reserved.
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|a Members of the MT-A70 family are key catalytic proteins involved in m6A methylation modifications in plants. They play diverse roles at the posttranscriptional level by regulating RNA secondary structure, selective splicing, stability, and translational efficiency, which collectively affect plant growth, development, and stress responses. In this study, we explored the function of the gene SlMTC, a Class C member of the MT-A70 family, in tomatoes by using CRISPR/Cas9 technology. Compared with the wild-type (WT), the CR-slmtc mutants exhibited decreased seed size and slower growth rates during the seedling stage, along with weaker salt tolerance and significant downregulation of stress-related genes, such as PR1, PR5, and P5CS. The qRT-PCR results revealed that the expression levels of genes involved in auxin biosynthesis (FZY1, FZY3, and FZY4) and polar transport (PIN1, PIN4, and PIN8) were lower in CR-slmtc plants than in the WT plants. In addition, yeast two-hybrid assays showed that SlMTC could interact with SlMTA, a Class A member of the MT-A70 family, providing insights into the potential mode of action of SlMTC in tomatoes. Overall, our findings indicate the critical role of SlMTC in plant growth and development as well as in response to salt stress
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|a Journal Article
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|a Salt stresses
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| 650 |
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|a Seed size
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|a SlMTC
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|a Tomato
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
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|a Yang, Qingling
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shen, Hui
|e verfasserin
|4 aut
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| 700 |
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|a Guo, Pengyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Qiaoli
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Guoping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hu, Zongli
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 349(2024) vom: 31. Nov., Seite 112228
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
1 |
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|g volume:349
|g year:2024
|g day:31
|g month:11
|g pages:112228
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| 856 |
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|u http://dx.doi.org/10.1016/j.plantsci.2024.112228
|3 Volltext
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