|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM377010707 |
| 003 |
DE-627 |
| 005 |
20240924232756.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
240902s2024 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2024.109079
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1547.xml
|
| 035 |
|
|
|a (DE-627)NLM377010707
|
| 035 |
|
|
|a (NLM)39213944
|
| 035 |
|
|
|a (PII)S0981-9428(24)00747-2
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Hou, Xuemei
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a SlNAP1 promotes tomato fruit ripening by regulating carbohydrate metabolism
|
| 264 |
|
1 |
|c 2024
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 14.09.2024
|
| 500 |
|
|
|a Date Revised 24.09.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2024 Elsevier Masson SAS. All rights reserved.
|
| 520 |
|
|
|a Many studies showed NAC transcription factors play an important role in fruit ripening. Moreover, sucrose and starch metabolism is also closely related to fruit ripening. However, there are a few studies focus on whether NAC regulates sucrose and starch metabolism to influence fruit ripening. In this study, virus-induced gene silencing (VIGS) of SlNAP1 suppressed fruit ripening and delayed color transformation. The chlorophyll (including Chla, Chlb, and Chla + b) degradation and carotenoid synthesis in SlNAP1-silenced fruits were dramatically suppressed. Silencing SlNAP1 decreased soluble sugar and reducing sugar accumulation in fruits, and increased starch content. The activity of starch degrading enzymes, including α amylase (AMY) and β amylase (BAM) was significantly lower in SlNAP1-silenced fruits than in the control fruits, whereas denosine diphosphoglucose pyrophosphorylase (AGP) activity was significantly higher. In addition, the expression of starch degradation-related genes (SlAMY1, SlAMY2, SlBAM1, SlBAM7, SlGWD, SlPWD) in SlNAP1-silenced fruits was significantly suppressed, while starch synthesis-related genes (SlAGPase1, SlAGPase2) was significantly increased. Compared with the control fruits, SlNAP1-silenced fruits showed significantly lower sucrose and glucose content. The expression level of sucrose and glucose metabolism-related genes such as Slsus1, Slsus3, SlSPS, SlHxk1, SlHxk2, SlPK1, and SlPK2 was significantly lower in SlNAP1-silenced fruits than in the control fruits. Overall, this study revealed that SlNAP1 gene might positively regulate fruit ripening by influencing carbohydrate metabolism
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Fruit ripening
|
| 650 |
|
4 |
|a NAC
|
| 650 |
|
4 |
|a Sugar
|
| 650 |
|
4 |
|a VIGS
|
| 650 |
|
7 |
|a Chlorophyll
|2 NLM
|
| 650 |
|
7 |
|a 1406-65-1
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Starch
|2 NLM
|
| 650 |
|
7 |
|a 9005-25-8
|2 NLM
|
| 650 |
|
7 |
|a Sucrose
|2 NLM
|
| 650 |
|
7 |
|a 57-50-1
|2 NLM
|
| 650 |
|
7 |
|a Transcription Factors
|2 NLM
|
| 650 |
|
7 |
|a Adenosine Triphosphatases
|2 NLM
|
| 650 |
|
7 |
|a EC 3.6.1.-
|2 NLM
|
| 700 |
1 |
|
|a Liu, Huwei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Yihua
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhang, Zhuohui
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Tong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Liang, Chen
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Chunlei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Changxia
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Liao, Weibiao
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 215(2024) vom: 23. Sept., Seite 109079
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
|
| 773 |
1 |
8 |
|g volume:215
|g year:2024
|g day:23
|g month:09
|g pages:109079
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2024.109079
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 215
|j 2024
|b 23
|c 09
|h 109079
|