TaNAC1 boosts powdery mildew resistance by phosphorylation-dependent regulation of TaSec1a and TaCAMTA4 via PP2Ac/CDPK20

Détails bibliographiques
Publié dans:	The New phytologist. - 1979. - 244(2024), 2 vom: 09. Okt., Seite 635-653
Auteur principal:	Liu, Yuanming (Auteur)
Autres auteurs:	You, Hongguang, Li, Hanping, Zhang, Chujun, Guo, Huan, Huang, Xueling, Zhang, Qiong, Zhang, Xiangyu, Ma, Chuang, Wang, Yajuan, Li, Tingdong, Ji, Wanquan, Kang, Zhensheng, Zhang, Hong
Format:	Article en ligne
Langue:	English
Publié:	2024
Accès à la collection:	The New phytologist
Sujets:	Journal Article TaNAC1 cell death phosphorylation powdery mildew resistance wheat Plant Proteins Protein Phosphatase 2 EC 3.1.3.16 Transcription Factors

Description
Résumé:	© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation. The integrity of wheat (Triticum aestivum) production is increasingly jeopardized by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), particularly amid the vicissitudes of climate change. Here, we delineated the role of a wheat transcription factor, TaNAC1, which precipitates cellular apoptosis and fortifies resistance against Bgt. Utilizing BiFC, co-immunoprecipitation, protein quantification, luciferase report assays, we determined that cytoplasmic TaNAC1-7A undergoes phosphorylation at the S184/S258 sites by TaCDPK20, facilitating its nuclear translocation. This migration appears to prime further phosphorylation by TaMPK1, thereby enhancing transcriptional regulatory activity. Notably, the apoptotic activity of phosphorylated TaNAC1-7A is negatively modulated by the nuclear protein phosphatase PP2Ac. Furthermore, activation of TaNAC1 phosphorylation initiates transcription of downstream genes TaSec1a and TaCAMTA4, through binding to the C[T/G]T[N7]A[A/C]G nucleic acid motif. Suppression of TaNAC1, TaCDPK20, and TaMPK1 in wheat compromises its resistance to Bgt strain E09, whereas overexpression of TaNAC1 and silencing of PP2Ac markedly elevate resistance levels. Our results reveal the pivotal role of TaNAC1 in basal resistance which is mediated by its effects on homotypic fusion, vacuolar protein sorting, and the expression of defense-related genes. The findings highlight the potential through targeting TaNAC1 and its regulators as a strategy for improving wheat's resistance to fungal pathogens
Description:	Date Completed 17.10.2024 Date Revised 28.10.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.20070