|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM376567902 |
| 003 |
DE-627 |
| 005 |
20250306133712.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
240822s2024 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erae303
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1254.xml
|
| 035 |
|
|
|a (DE-627)NLM376567902
|
| 035 |
|
|
|a (NLM)39169567
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Ngo, Anh H
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Non-specific phospholipase C3 is involved in endoplasmic reticulum stress tolerance in Arabidopsis
|
| 264 |
|
1 |
|c 2024
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 30.10.2024
|
| 500 |
|
|
|a Date Revised 27.11.2024
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
|
| 520 |
|
|
|a Non-specific phospholipase C (NPC) is an emerging family of lipolytic enzymes unique to plants and bacteria that play crucial roles in growth and stress responses. Among six copies of NPC isoforms found in Arabidopsis, the role of NPC3 remains elusive to date. Here, we show that NPC3 is a functional non-specific phospholipase C involved in tolerance to tunicamycin (TM)-induced endoplasmic reticulum (ER) stress through the synthesis of phosphocholine (PCho), a reaction product of NPC3. The npc3 mutant exhibited reduced sensitivity to TM treatment. Recombinant NPC3 possessed pronounced phospholipase C activity that hydrolyses phosphatidylcholine (PC). The hyposensitivity of npc3 to TM treatment was complemented by exogenous PCho, suggesting that NPC3-catalysed PCho production is involved in TM-induced ER stress tolerance. NPC3 was localized at the ER and was predominantly expressed in the roots, and it was further induced by TM-induced ER stress. Intriguingly, npc3 mutants showed a markedly reduced PCho content in shoots under ER stress. Our results indicate that ER stress induces NPC3 to produce PCho, which is involved in TM-induced ER stress tolerance
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Arabidopsis thaliana
|
| 650 |
|
4 |
|a ER stress
|
| 650 |
|
4 |
|a endoplasmic reticulum (ER)
|
| 650 |
|
4 |
|a non-specific phospholipase C (NPC)
|
| 650 |
|
4 |
|a phosphocholine (PCho)
|
| 650 |
|
4 |
|a phospholipids
|
| 650 |
|
4 |
|a unfolded protein response (UPR)
|
| 650 |
|
7 |
|a Arabidopsis Proteins
|2 NLM
|
| 650 |
|
7 |
|a Tunicamycin
|2 NLM
|
| 650 |
|
7 |
|a 11089-65-9
|2 NLM
|
| 650 |
|
7 |
|a Type C Phospholipases
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.4.-
|2 NLM
|
| 650 |
|
7 |
|a Phosphorylcholine
|2 NLM
|
| 650 |
|
7 |
|a 107-73-3
|2 NLM
|
| 700 |
1 |
|
|a Angkawijaya, Artik Elisa
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Nakamura, Yuki
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kanehara, Kazue
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 20 vom: 30. Okt., Seite 6489-6499
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
|
| 773 |
1 |
8 |
|g volume:75
|g year:2024
|g number:20
|g day:30
|g month:10
|g pages:6489-6499
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erae303
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 75
|j 2024
|e 20
|b 30
|c 10
|h 6489-6499
|