Polymeric Nanocarriers Autonomously Cross the Plant Cell Wall and Enable Protein Delivery for Stress Sensing

Bibliographische Detailangaben
Veröffentlicht in:	Advanced materials (Deerfield Beach, Fla.). - 1998. - 36(2024), 41 vom: 01. Okt., Seite e2409356
1. Verfasser:	Zhang, Yilin (VerfasserIn)
Weitere Verfasser:	Cao, Yunteng, Jiang, Wenzhi, Ma, Qingquan, Shin, Jinwoo, Sun, Hui, Cui, Jianqiao, Chen, Yongsheng, Giraldo, Juan Pablo, Strano, Michael S, Lowry, Gregory V, Sheen, Jen, Marelli, Benedetto
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Advanced materials (Deerfield Beach, Fla.)
Schlagworte:	Journal Article nanocarrier plant engineering polymers protein delivery stress sensing Polymers Green Fluorescent Proteins 147336-22-9 Drug Carriers

Beschreibung
Zusammenfassung:	© 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH. Delivery of proteins in plant cells can facilitate the design of desired functions by modulation of biological processes and plant traits but is currently limited by narrow host range, tissue damage, and poor scalability. Physical barriers in plants, including cell walls and membranes, limit protein delivery to desired plant tissues. Herein, a cationic high aspect ratio polymeric nanocarriers (PNCs) platform is developed to enable efficient protein delivery to plants. The cationic nature of PNCs binds proteins through electrostatic. The ability to precisely design PNCs' size and aspect ratio allowed us to find a cutoff of ≈14 nm in the cell wall, below which cationic PNCs can autonomously overcome the barrier and carry their cargo into plant cells. To exploit these findings, a reduction-oxidation sensitive green fluorescent protein (roGFP) is deployed as a stress sensor protein cargo in a model plant Nicotiana benthamiana and common crop plants, including tomato and maize. In vivo imaging of PNC-roGFP enabled optical monitoring of plant response to wounding, biotic, and heat stressors. These results show that PNCs can be precisely designed below the size exclusion limit of cell walls to overcome current limitations in protein delivery to plants and facilitate species-independent plant engineering
Beschreibung:	Date Completed 10.10.2024 Date Revised 12.10.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-4095
DOI:	10.1002/adma.202409356