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240814s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2024.109026
|2 doi
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|a pubmed24n1533.xml
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|a (DE-627)NLM376250321
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|a (NLM)39137685
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|a (PII)S0981-9428(24)00694-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Jiang, Yang
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Regulation of ginseng adventitious root growth in Panax ginseng by the miR156-targeted PgSPL24-09 transcription factors
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|c 2024
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.09.2024
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|a Date Revised 14.09.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier Masson SAS. All rights reserved.
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|a MicroRNA (miRNA) is a class of non-coding endogenous small-molecule, single-stranded RNAs, and it is involved in post-transcriptional gene expression regulation in plants and plays an important role in plant growth and development. Among them, miRNA156 regulates members of target SPL gene family and thus participates in plant growth and development, hormonal response and adversity stress. However, it has not been reported in ginseng. In this study, based on the previous analysis of the SPL gene family, the age-related and stably expressed SPL gene PgSPL24-09 was obtained in roots. The binding site of miRNA156 to this gene was analyzed using target gene prediction tools, and the interactions between miRNA156 and PgSPL24-09 gene were verified by dual luciferase reporter gene assay and RT-qPCR. At the same time, miRNA156 silencing vector and overexpression vector were constructed and transformed into ginseng adventitious roots and Arabidopsis thaliana to analyze the molecular mechanism of miRNA156-SPL module in regulating the growth of ginseng adventitious roots. This study provides a theoretical basis for the in-depth study of the molecular role of miRNAs in ginseng growth, and also lays the foundation for the study of the role of miRNA156-SPL module in regulating the growth and development of ginseng
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|a Journal Article
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|a Ginseng adventitious root
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| 650 |
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|a Growth and development
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| 650 |
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|a Panax ginseng
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4 |
|a SPL gene
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| 650 |
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4 |
|a miRNA156
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| 650 |
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|a MicroRNAs
|2 NLM
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| 650 |
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7 |
|a Transcription Factors
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a RNA, Plant
|2 NLM
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| 700 |
1 |
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|a Liu, Chang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a He, Gaohui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Yu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Mengna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Kexin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Mingming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Aimin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Meiping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Mingzhu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Kangyu
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 215(2024) vom: 01. Sept., Seite 109026
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
8 |
|g volume:215
|g year:2024
|g day:01
|g month:09
|g pages:109026
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2024.109026
|3 Volltext
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|d 215
|j 2024
|b 01
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|h 109026
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