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|a 10.1093/jxb/erae337
|2 doi
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|a pubmed24n1616.xml
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Pinoti, Vitor F
|e verfasserin
|4 aut
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|a SCI1, a flower regulator of cell proliferation, and its partners NtCDKG2 and NtRH35 interact with the splicing machinery
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|c 2024
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 30.10.2024
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|a Date Revised 27.11.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
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|a Successful plant reproduction depends on the adequate development of floral organs controlled by cell proliferation and other processes. The Stigma/style cell-cycle inhibitor 1 (SCI1) gene regulates cell proliferation and affects the final size of the female reproductive organ. To unravel the molecular mechanism exerted by Nicotiana tabacum SCI1 in cell proliferation control, we searched for its interaction partners through semi-in vivo pull-down experiments, uncovering a cyclin-dependent kinase, NtCDKG;2. Bimolecular fluorescence complementation and co-localization experiments showed that SCI1 interacts with NtCDKG;2 and its cognate NtCyclin L in nucleoli and splicing speckles. The screening of a yeast two-hybrid cDNA library using SCI1 as bait revealed a novel DEAD-box RNA helicase (NtRH35). Interaction between the NtCDKG;2-NtCyclin L complex and NtRH35 is also shown. Subcellular localization experiments showed that SCI1, NtRH35, and the NtCDKG;2-NtCyclin L complex associate with each other within splicing speckles. The yeast two-hybrid screening of NtCDKG;2 and NtRH35 identified the conserved spliceosome components U2a', NF-κB activating protein (NKAP), and CACTIN. This work presents SCI1 and its interactors, the NtCDKG;2-NtCyclin L complex and NtRH35, as new spliceosome-associated proteins. Our findings reveal a network of interactions and indicate that SCI1 may regulate cell proliferation through the splicing process, providing new insights into the intricate molecular pathways governing plant development
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|a Journal Article
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|a C-terminal domain of RNA polymerase II (Pol II-CTD)
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|a CACTIN
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|a CDKG;2
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|a Cyclin L
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|a DEAD-box RNA helicase RH35
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|a NF-κB activating protein (NKAP)
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|a RPB11
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|a RPB7
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|a U2aʹ
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|a spliceosome
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|a Plant Proteins
|2 NLM
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|a DEAD-box RNA Helicases
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|a EC 3.6.4.13
|2 NLM
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| 700 |
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|a Ferreira, Pedro B
|e verfasserin
|4 aut
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|a Strini, Edward J
|e verfasserin
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|a Lubini, Greice
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|a Thomé, Vanessa
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|a Cruz, Joelma O
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|a Aziani, Rodrigo
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|a Quiapim, Andréa C
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|a Pinto, Andressa P A
|e verfasserin
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|a Araujo, Ana Paula U
|e verfasserin
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|a De Paoli, Henrique C
|e verfasserin
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|a Pranchevicius, Maria Cristina S
|e verfasserin
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|a Goldman, Maria Helena S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 75(2024), 20 vom: 30. Okt., Seite 6312-6330
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:75
|g year:2024
|g number:20
|g day:30
|g month:10
|g pages:6312-6330
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|u http://dx.doi.org/10.1093/jxb/erae337
|3 Volltext
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